Planococcus versutus sp. nov., isolated from soil

See-Too, Wah-Seng; Ee, Robson; Madhaiyan, Munusamy; Kwon, Soon-Wo; Tan, Jua Yi; Lim, Yan Lue; Convey, Peter ORCID: https://orcid.org/0000-0001-8497-9903; Pearce, David ORCID: https://orcid.org/0000-0001-5292-4596; Yin, Wai-Fong; Chan, Kok-Gan. 2017 Planococcus versutus sp. nov., isolated from soil. International Journal of Systematic and Evolutionary Microbiology, 67 (4). 944-950. 10.1099/ijsem.0.001721

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Abstract/Summary

A taxonomic study was performed on a novel Gram-stain-positive, coccus-shaped, orange-pigmented motile bacterium, designated as strain L10.15T. The organism was isolated from a soil sample collected in Lagoon Island (close to Adelaide Island, western Antarctic Peninsula) using a quorum-quenching enrichment medium. Growth occurred at 4–30 °C, pH 6–11 and at moderately high salinity (0–15 %, w/v, NaCl), with optimal growth at 26 °C, at pH 7–8 and with 6 % (w/v) NaCl. 16S rRNA gene sequence analysis showed that strain L10.15T belonged to the genus Planococcus and was closely related to Planococcus halocryophilus Or1T (99.3 % similarity), Planococcus donghaensis JH1T (99.0 %), Planococcus antarcticus DSM 14505T (98.3 %), Planococcus plakortidis AS/ASP6 (II)T (97.6 %), Planococcus maritimus TF-9T (97.5 %), Planococcus salinarum ISL-6T (97.5 %) and Planococcus kocurii NCIMB 629T (97.5 %). However, the average nucleotide identity-MUMmer analysis showed low genomic relatedness values of 71.1–81.7 % to the type strains of these closely related species of the genus Planococcus . The principal fatty acids were anteiso-C15 : 0, C16 : 1ω7c and anteiso-C17 :  0, and the major menaquinones of strain L10.15T were MK-5 (48 %), MK-6 (6 %) and MK-7 (44 %). Polar lipid analysis revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and aminophospholipid. The DNA G+C content was 39.4 mol%. The phenotypic and genotypic data indicate that strain L10.15T represents a novel species of the genus Planococcus , for which the name Planococcus versutus sp. nov. is proposed. The type strain is L10.15T (=DSM 101994T=KACC 18918T).

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1099/ijsem.0.001721
Programmes:	BAS Programmes > BAS Programmes 2015 > Biodiversity, Evolution and Adaptation
Date made live:	27 Jun 2017 10:45 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/514158

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