Application of novel image base estimation of invisible leaf injuries in relation to morphological and photosynthetic changes of Phaseolus vulgaris L. exposed to tropospheric ozone

Drapikowska, M.; Drapikowski, P.; Borowiak, K.; Hayes, F. ORCID: https://orcid.org/0000-0002-1037-5725; Harmens, H. ORCID: https://orcid.org/0000-0001-8792-0181; Dziewiatka, T.; Byczkowska, K.. 2016 Application of novel image base estimation of invisible leaf injuries in relation to morphological and photosynthetic changes of Phaseolus vulgaris L. exposed to tropospheric ozone. Atmospheric Pollution Research, 7 (6). 1065-1071. 10.1016/j.apr.2016.06.008

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Official URL: http://dx.doi.org/10.1016/j.apr.2016.06.008

Abstract/Summary

This study aimed to evaluate the degree of Phaseolus vulgaris L. (bean) leaf tissue injury caused by tropospheric ozone. To validate O3 symptoms at the microscopic level, Evans blue staining together with an image processing method for the removal of distortions and calculation of dead leaf areas was applied. Net photosynthetic rate (PN), stomatal conductance (gs) and intercellular CO2 concentration (Ci) were determined to evaluate leaf physiological responses to ozone. It was found that both resistant and sensitive varieties of bean were damaged by ozone; however, the size of necrotic and partially destroyed leaf area in the sensitive genotype (S156) was bigger (1.18%, 2.18%) than in the resistant genotype (R123), i.e. 0.02% and 0.50%. Values of net photosynthetic rates were lower in the sensitive genotype in ambient air conditions, than in the resistant genotype in ambient air conditions. We further found that there was a correlation between physiological and anatomical injuries; net photosynthetic rate (PN) was negatively correlated with percentage of necrotic area of both genotypes, while stomatal conductance (gs), intercellular CO2 concentration (Ci) were positively correlated with percentage of necrotic tissue of both genotypes. Moreover, visible injures in both genotypes were positively correlated with percentage of anatomical injures. In conclusion, the presented combinations of morphological, anatomical and physiological markers allowed differential diagnosis of ozone injury.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1016/j.apr.2016.06.008
UKCEH and CEH Sections/Science Areas:	Emmett
ISSN:	1309-1042
Additional Keywords:	tropospheric ozone, leaf injuries, image analysis, photosynthetic activity
NORA Subject Terms:	Ecology and Environment Atmospheric Sciences Botany
Related URLs:	Project Website
Date made live:	13 Jul 2016 11:04 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/513944

Item Type:

Publication - Article

Digital Object Identifier (DOI):

10.1016/j.apr.2016.06.008

UKCEH and CEH Sections/Science Areas:

Emmett

ISSN:

1309-1042

Additional Keywords:

tropospheric ozone, leaf injuries, image analysis, photosynthetic activity

NORA Subject Terms:

Ecology and Environment
Atmospheric Sciences
Botany