Differences in photosynthesis and terpene content in leaves and roots of wild-type and transgenic Arabidopsis thaliana plants
Blanch, J.S.; Penuelas, J.; Llusia, J.; Sardans, J.; Owen, S.M.. 2015 Differences in photosynthesis and terpene content in leaves and roots of wild-type and transgenic Arabidopsis thaliana plants. Russian Journal of Plant Physiology, 62 (6). 823-829. 10.1134/S1021443715060035
Before downloading, please read NORA policies.Preview |
Text
N511440PP.pdf - Accepted Version Download (360kB) | Preview |
Abstract/Summary
We investigated the hypotheses that two different varieties of Arabidopsis thaliana show differences in physiology and terpene production. The two varieties of A. thaliana used in this study were wild-type (WT) and transgenic line (CoxIV-FaNES I) genetically modified to emit nerolidol with linalool/nerolidol synthase (COX). Photosynthetic rate, electron transport rate, fluorescence, leaf volatile terpene contents and root volatile terpene contents were analyzed. For both types, we found co-eluting α-pinene+β-ocimene, limonene, and humulene in leaves; and in the roots we found co-eluting α-pinene+β-ocimene, sabinene+β-pinene, β-myrcene, limonene, and humulene. At the end of the growing cycle, COX plants tended to have lower pools of terpene compounds in their leaves, with 78.6% lower photosynthesis rates and 30.8% lower electron transport rates, compared with WT plants at that time. The maximal photochemical efficiency Fv/Fm was also significantly lower (25.5%) in COX plants, indicating that these varieties were more stressed than WT plants. However, COX plants had higher (239%) root terpene contents compared to WT plants. COX plants appear to favor root production of volatile terpenes rather than leaf production. Thus we conclude that there were significant differences between COX and WT plants in terms of terpenoid pools, stress status and physiology.
Item Type: | Publication - Article |
---|---|
Digital Object Identifier (DOI): | 10.1134/S1021443715060035 |
UKCEH and CEH Sections/Science Areas: | Dise |
ISSN: | 1021-4437 |
Additional Keywords: | Arabidopsis thaliana, FaNES I, leaf terpene contents, root terpene contents, photosynthesis |
NORA Subject Terms: | Ecology and Environment Atmospheric Sciences |
Date made live: | 12 Nov 2015 14:45 +0 (UTC) |
URI: | https://nora.nerc.ac.uk/id/eprint/511440 |
Actions (login required)
View Item |
Document Downloads
Downloads for past 30 days
Downloads per month over past year