Cross-amplification and characterization of polymorphic microsatellite markers from Acacia (Senegalia) mellifera and Acacia brevispica to Acacia senegal (L.) Willd.

Omondi, S.F.; Dangasuk, O.G.; Odee, D.W.; Cavers, S.; Khasa, D.P.

NORA

Cross-amplification and characterization of polymorphic microsatellite markers from Acacia (Senegalia) mellifera and Acacia brevispica to Acacia senegal (L.) Willd.

Omondi, S.F.; Dangasuk, O.G.; Odee, D.W.; Cavers, S. ORCID: https://orcid.org/0000-0003-2139-9236; Khasa, D.P.. 2010 Cross-amplification and characterization of polymorphic microsatellite markers from Acacia (Senegalia) mellifera and Acacia brevispica to Acacia senegal (L.) Willd. Silvae Genetica, 59. 285-288.

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Abstract

Seven polymorphic microsatellite markers isolated from Acacia brevispica and Acacia mellifera were successfully cross-amplified in Acacia senegal. The loci were surveyed for polymorphism using 30 samples. Allelic diversity ranged from 4 (Ame02, Ab06 and Ab18) to 13 (Ab26) per locus. The expected heterozygosity (HE) ranged from 0.543 (Ame02) to 0.868 (Ab26) while observed heterozygosity (HO) ranged from 0.516 (Ame05) to 0.800 (Ame03). Cross amplification of these loci represents a potential source of co-dominant marker and will be useful in the study of genetic diversity, structure, gene flow and breeding systems of this important Acacia species.

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