Enhanced biotransformation of TCE using plant terpenoids in contaminated groundwater

Brown, J.R.-M.; Thompson, I.P.; Paton, G.I.; Singer, A.C. ORCID: https://orcid.org/0000-0003-4705-6063. 2009 Enhanced biotransformation of TCE using plant terpenoids in contaminated groundwater. Letters in Applied Microbiology, 49 (6). 769-774. 10.1111/j.1472-765X.2009.02738.x

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Abstract/Summary

Aims: To examine plant terpenoids as inducers of TCE biotransformation by an indigenous microbial community originating from a plume of TCE-contaminated groundwater. Methods and Results: One-litre microcosms of groundwater were spiked with 100 mol 1-1 of TCE and amended weekly for 16 weeks with 20 l 1-1 of the following plant monoterpenes: linalool, pulegone, R- (+)-carvone, S- (-)carvone, farnesol, cumene. Yeast extract- and un-amended control treatments were also prepared. The addition of R- and S-carvone, linalool and cumene resulted in the biotransformation of upwards of 88 % of the TCE, significantly more than the unamendment control (61 %). The aforementioned group of terpenes also significantly (P < 0.05) allowed more TCE to be degraded than the remaining two terpenes (farnesol and pulegone) and the yeast extract treatment which biotransformed 74-75 % of the TCE. The microbial community profile was monitored by denaturing gradient gel electrophoresis and demonstrated much greater similarities between the microbial communities in terpene-amended treatments than in the yeast extract or unamended controls. Conclusions: TCE biotransformation can be signficantly enhanced through the addition of selected plant terpenoids. Significance and Impact of Study: Plant terpenoid and nutrient supplementation to groundwater might provide an environmentally benign means of enhancing the rate of in situ TCE bioremediation.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1111/j.1472-765X.2009.02738.x
Programmes:	CEH Programmes pre-2009 publications > Biogeochemistry > SE01B Sustainable Monitoring, Risk Assessment and Management of Chemicals CEH Topics & Objectives 2009 - 2012 > Biogeochemistry > BGC Topic 3 - Managing Threats to Environment and Health
UKCEH and CEH Sections/Science Areas:	Hails
ISSN:	0266-8254
Additional Keywords:	Secondary plant metabolites, biostimulation, TCE, groundwater, plant terpenes, carvone, cumene
NORA Subject Terms:	Biology and Microbiology
Date made live:	26 Jan 2010 11:22 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/8098

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