Mikryukov, Vladimir
ORCID: https://orcid.org/0000-0003-2786-2690; Dulya, Olesya
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ORCID: https://orcid.org/0000-0001-5526-4845; Anslan, Sten
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ORCID: https://orcid.org/0000-0003-0616-5829; Prins, Victoria
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ORCID: https://orcid.org/0000-0003-4743-6111; Põlme, Sergei
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ORCID: https://orcid.org/0000-0002-8585-3429; Bahram, Mo
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ORCID: https://orcid.org/0000-0002-8810-8838; Agan, Ahto; Ahmed, Talaat
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ORCID: https://orcid.org/0000-0001-5084-850X; Albornoz, Felipe E.
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ORCID: https://orcid.org/0000-0001-7381-5110; Alvarez-Manjarrez, Julieta
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ORCID: https://orcid.org/0000-0002-8120-5248; Bauters, Marijn
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ORCID: https://orcid.org/0000-0001-5053-5693; Bråthen, Kari Anne
ORCID: https://orcid.org/0000-0003-0942-1074; Buegger, Franz
ORCID: https://orcid.org/0000-0003-3526-4711; Butterbach-Bahl, Klaus
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ORCID: https://orcid.org/0000-0003-0499-8536; Cameron, Erin K.
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ORCID: https://orcid.org/0000-0002-9626-6318; Casique-Valdés, Rebeca
ORCID: https://orcid.org/0000-0002-0497-8255; Corrales, Adriana
ORCID: https://orcid.org/0000-0001-9885-4634; Davydov, Evgeny A.
ORCID: https://orcid.org/0000-0002-2316-8506; De Crop, Eske
ORCID: https://orcid.org/0000-0002-9067-6981; De Kesel, André
ORCID: https://orcid.org/0000-0002-5287-4637; Djeugap, Joseph Fovo
ORCID: https://orcid.org/0000-0003-4786-7026; Drenkhan, Rein; Duarte Ritter, Camila
ORCID: https://orcid.org/0000-0002-3371-7425; Dudov, Sergey V.
ORCID: https://orcid.org/0000-0003-1512-0956; Espenberg, Mikk
ORCID: https://orcid.org/0000-0003-0469-6394; Fanuel, Ongua
ORCID: https://orcid.org/0009-0002-4902-4858; Fedosov, Vladimir E.
ORCID: https://orcid.org/0000-0002-5331-6346; Florence, Luke
ORCID: https://orcid.org/0000-0002-1901-7772; Furneaux, Brendan R.
ORCID: https://orcid.org/0000-0003-3522-7363; Furtado, Ariadne N. M.
ORCID: https://orcid.org/0000-0001-9596-7553; Färkkilä, Sanni
ORCID: https://orcid.org/0000-0002-4485-5567; Gamova, Natalia S.
ORCID: https://orcid.org/0000-0002-4141-757X; Garibay-Orijel, Roberto
ORCID: https://orcid.org/0000-0002-6977-7550; Geml, József
ORCID: https://orcid.org/0000-0001-8745-0423; Ghosh, Soumya
ORCID: https://orcid.org/0000-0002-4945-3516; Godoy, Roberto
ORCID: https://orcid.org/0000-0002-3719-3091; Gohar, Daniyal
ORCID: https://orcid.org/0000-0003-0312-1142; Gryzenhout, Marieka; Hasan, Ayad H.
ORCID: https://orcid.org/0000-0001-7280-2254; Hashem, Amr H.; Heilmann-Clausen, Jacob
ORCID: https://orcid.org/0000-0003-4713-6004; Henkel, Terry W.
ORCID: https://orcid.org/0000-0001-9760-8837; Hiiesalu, Indrek; Hiiesalu, Inga
ORCID: https://orcid.org/0000-0002-5457-2376; Hosseyni Moghaddam, Mahdieh S.; Hyde, Kevin D.; Inostroza, Karina
ORCID: https://orcid.org/0000-0003-3026-6291; Kariman, Khalil
ORCID: https://orcid.org/0000-0002-2070-4713; Karimullina, Elina
ORCID: https://orcid.org/0000-0001-6459-2293; Kepfer-Rojas, Sebastian
ORCID: https://orcid.org/0000-0002-1681-2877; Khalid, Abdul Nasir; Klavina, Darta
ORCID: https://orcid.org/0000-0002-1455-9062; Kohout, Petr
ORCID: https://orcid.org/0000-0002-3985-2310; Korotkov, Yuri N.
ORCID: https://orcid.org/0000-0001-8436-0887; Kupagme, John Y.
ORCID: https://orcid.org/0000-0002-9981-050X; Kurina, Olavi
ORCID: https://orcid.org/0000-0002-4858-4629; Lamit, Louis James
ORCID: https://orcid.org/0000-0002-0385-6010; Lateef, Adebola Azeez; Ledoux, Njouonkou André
ORCID: https://orcid.org/0000-0001-9733-7248; Lim, Young Woon
ORCID: https://orcid.org/0000-0003-2864-3449; Maciá-Vicente, Jose G.; Makovskis, Kristaps
ORCID: https://orcid.org/0000-0003-4943-1912; Martínez, Sebastián
ORCID: https://orcid.org/0000-0003-0455-5823; Marín, César
ORCID: https://orcid.org/0000-0002-2529-8929; Meidl, Peter; Mortimer, Peter E.; Mundra, Sunil
ORCID: https://orcid.org/0000-0002-0535-118X; Naluyange, Victoria
ORCID: https://orcid.org/0000-0002-3990-9751; Netherway, Tarquin
ORCID: https://orcid.org/0000-0002-9049-9225; Newsham, Kevin K.
ORCID: https://orcid.org/0000-0002-9108-0936; Nouhra, Eduardo
ORCID: https://orcid.org/0000-0002-7080-8211; Nyamukondiwa, Casper; Nteziryayo, Vincent
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ORCID: https://orcid.org/0000-0003-3985-9421; Oja, Jane
ORCID: https://orcid.org/0000-0003-1446-2284; Onipchenko, Vladimir G.
ORCID: https://orcid.org/0000-0002-1626-1171; Otsing, Eveli
ORCID: https://orcid.org/0000-0001-7416-257X; Owaid, Mustafa Nadhim
ORCID: https://orcid.org/0000-0001-9005-4368; Piepenbring, Meike
ORCID: https://orcid.org/0000-0002-7043-5769; Pochekutova, Polina
ORCID: https://orcid.org/0009-0001-0453-9869; Pombo, Maihyra Marina
ORCID: https://orcid.org/0000-0002-0329-9736; Pritsch, Karin
ORCID: https://orcid.org/0000-0001-6384-2473; Puusepp, Rasmus
ORCID: https://orcid.org/0000-0002-0617-3776; Pärn, Jaan
ORCID: https://orcid.org/0000-0001-6507-8894; Põldmaa, Kadri
ORCID: https://orcid.org/0000-0002-7936-2455; Rahimlou, Saleh
ORCID: https://orcid.org/0000-0003-0427-1329; Rinaldi, Andrea C.
ORCID: https://orcid.org/0000-0002-9352-1037; Rojas, Oscar
ORCID: https://orcid.org/0000-0002-0176-9685; Roslin, Tomas
ORCID: https://orcid.org/0000-0002-2957-4791; Runnel, Kadri
ORCID: https://orcid.org/0000-0002-7308-3623; Rähn, Elisabeth; Saba, Malka
ORCID: https://orcid.org/0000-0001-7673-2345; Saitta, Alessandro; Salih, Talal Sabhan
ORCID: https://orcid.org/0000-0001-8345-6131; Sarapuu, Joosep
ORCID: https://orcid.org/0000-0002-4805-5612; Serrano, Eduard
ORCID: https://orcid.org/0000-0001-9082-473X; Serrano, Oscar
ORCID: https://orcid.org/0000-0002-5973-0046; Sharmah, Dipon
ORCID: https://orcid.org/0000-0002-5579-3103; Sharp, Cathy
ORCID: https://orcid.org/0009-0003-4985-1543; Skalska-Tuomi, Maria W.
ORCID: https://orcid.org/0000-0002-7154-5177; Tchan, Kassim Issifou
ORCID: https://orcid.org/0000-0002-8572-3405; Truong, Camille
ORCID: https://orcid.org/0000-0002-8510-1761; van der Merwe, Helga
ORCID: https://orcid.org/0000-0002-7677-5123; Vanié-Léabo, Linda L. P.
ORCID: https://orcid.org/0000-0001-6504-3456; Vasco-Palacios, Aida M.
ORCID: https://orcid.org/0000-0003-0539-9711; Verbeken, Annemieke; Vlk, Lukáš
ORCID: https://orcid.org/0000-0001-9201-8715; Wijayawardene, Nalin N.
ORCID: https://orcid.org/0000-0003-0522-5498; Wood, Jennifer L.
ORCID: https://orcid.org/0000-0002-7313-5681; Yasanthika, W. A. Erandi
ORCID: https://orcid.org/0000-0002-3757-3801; Yorou, Nourou S.
ORCID: https://orcid.org/0000-0001-6997-811X; Zahn, Geoffrey
ORCID: https://orcid.org/0000-0002-8691-692X; Zettur, Irma; Zucconi, Laura
ORCID: https://orcid.org/0000-0001-9793-2303; Kõljalg, Urmas
ORCID: https://orcid.org/0000-0002-5171-1668; Tedersoo, Leho
ORCID: https://orcid.org/0000-0002-1635-1249.
2026
Global dataset of soil eukaryotic communities created with a uniform protocol and long read sequencing.
Scientific Data, 13.
12, pp.
10.1038/s41597-026-07315-y
Soil eukaryotes, including fungi, protists, plants, and animals, are central to biosphere functioning and resilience. The Global Standardised Soil Eukaryome Dataset (GloSED) is the first dataset encompassing the entire spectrum of soil eukaryotes, covering 4,063 sampling sites in 121 countries on all continents, revealing nearly one million operational taxonomic units. All samples were collected and analysed using a standardised protocol minimizing technical biases. Long-read sequencing of full-length ITS and 18S-V9 regions provide broad taxonomic coverage and high-resolution identification supported by specialist curation of “dark taxa”. A rigorous bioinformatic processing ensures against homopolymer errors, PCR-mediated chimeras, and index switching providing high data quality. The dataset is supported by raw sequences and an open-source containerised workflow for reproducible analyses. The samples are accompanied by land-cover description and directly measured soil pH, δ 13 C, δ 15 N, as well as P, K, Ca, Mg, and total C and N contents. GloSED is the first database that enables ecological and biogeographic studies of entire soil eukaryotic communities from local to global scales.
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