Detection of Genes Encoding for Absorption of Hydrocarbons Degradation by Bacteria Isolated from Antarctica, Malaysia, and Indonesia

Allafi, Faisal ORCID: https://orcid.org/0000-0002-1340-3656; Ridwan, Muhammad; Convey, Peter ORCID: https://orcid.org/0000-0001-8497-9903; Wijaya, Dani; Merican, Faradina; Lalung, Japareng ORCID: https://orcid.org/0000-0001-5134-6456. 2025 Detection of Genes Encoding for Absorption of Hydrocarbons Degradation by Bacteria Isolated from Antarctica, Malaysia, and Indonesia. Next Research, 2 (3), 100558. 6, pp. 10.1016/j.nexres.2025.100558

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Abstract/Summary

Petroleum spills pose a significant threat to ecosystems due to the presence of hydrocarbons, the primary toxic components. Bioremediation, particularly using hydrocarbon-degrading bacteria, offers an eco-friendly solution for mitigating this pollution. The present study aims to detect genes specifically the alkB gene associated with hydrocarbon degradation in bacterial strains isolated from oil-contaminated environments. Bacteria were successfully isolated from two distinct locations, Antarctica and Pulau Pinang. Molecular identification using 16S rRNA gene sequencing revealed the isolates as Brevibacterium casei with 92.85% similarity. The detection of the alkB gene in these strains suggests their potential use in cost-effective and efficient bioremediation strategies for hydrocarbon-contaminated environments.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1016/j.nexres.2025.100558
ISSN:	30504759
Additional Keywords:	alkB gene, hydrocarbon-degrading bacteria, Antarctica bioremediation, oil-contaminated water, comparative microbial analysis
Date made live:	02 Jul 2025 14:04 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/539763

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