Visualisation of Mycobacterium avium subsp. paratuberculosis in cultured cells, infected sheep and human tissue sections using fluorescent in situ hybridization (FISH)

Rayment, Neil; Rhodes, Glenn ORCID: https://orcid.org/0000-0003-0488-3843; Hudspith, Barry; Hughes, Valerie; Chianini, Francesca; Agrawal, Gaurav; Bull, Tim J.; Pickup, Roger; Sanderson, Jeremy. 2024 Visualisation of Mycobacterium avium subsp. paratuberculosis in cultured cells, infected sheep and human tissue sections using fluorescent in situ hybridization (FISH). Journal of Microbiological Methods, 224, 107001. 3, pp. 10.1016/j.mimet.2024.107001

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Official URL: http://dx.doi.org/10.1016/j.mimet.2024.107001

Abstract/Summary

We describe the development, testing and specificity of a modified oligonucleotide probe for the specific detection of Mycobacterium avium subsp. paratuberculosis (MAP) in culture and in infected tissue using fluorescent in situ hybridisation and confocal microscopy. The detection of MAP in both animal and human tissue using our modified probe allows for a more rapid diagnosis of MAP infection compared to the more often applied detection methods of culture and PCR and has the potential for quantification of cellular abundance. This approach would enable earlier treatment intervention and therefore the potential for reduced morbidity.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1016/j.mimet.2024.107001
UKCEH and CEH Sections/Science Areas:	Water Resources (Science Area 2017-)
ISSN:	0167-7012
Additional Information. Not used in RCUK Gateway to Research.:	Open Access paper - full text available via Official URL link.
Additional Keywords:	Mycobacterium avium ssp. paratuberculosis (MAP), FISH, Crohn's disease, Johne's disease
NORA Subject Terms:	Medicine Biology and Microbiology
Date made live:	23 Jul 2024 15:19 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/537754

Item Type:

Publication - Article

Digital Object Identifier (DOI):

10.1016/j.mimet.2024.107001

UKCEH and CEH Sections/Science Areas:

Water Resources (Science Area 2017-)

ISSN:

0167-7012

Additional Information. Not used in RCUK Gateway to Research.:

Open Access paper - full text available via Official URL link.

Additional Keywords:

Mycobacterium avium ssp. paratuberculosis (MAP), FISH, Crohn's disease, Johne's disease