Biocontrol mechanisms of the Antarctic yeast Debaryomyces hansenii UFT8244 against post-harvest phytopathogenic fungi of strawberries

Ferreira, Eskálath Morganna S.; Garmendia, Gabriela; Gonçalves, Vívian N.; da Silva, Juliana F.M.; Convey, Peter ORCID: https://orcid.org/0000-0001-8497-9903; Rosa, Luiz Henrique; Vero, Silvana; Pimenta, Raphael S.. 2024 Biocontrol mechanisms of the Antarctic yeast Debaryomyces hansenii UFT8244 against post-harvest phytopathogenic fungi of strawberries. Anais da Academia Brasileira de Ciências, 96 (Suppl. 2), e20240255. 11, pp. 10.1590/0001-3765202420240255

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Abstract/Summary

The use of yeasts has been explored as an efficient alternative to fungicide application in the treatment and prevention of post-harvest fruit deterioration. Here, we evaluated the biocontrol abilities of the Antarctic yeast strain Debaryomyces hansenii UFT8244 against the post-harvest phytopathogenic fungi Botrytis cinerea and Rhizopus stolonifer for the protection and preservation of strawberry fruit. The strongest inhibition of germination of B. cinerea (57%) was observed at 0 °C, followed by 40% at 25 °C. In addition, germ tubes and hyphae of B. cinerea were strongly surrounded and colonized by D. hansenii. Production of the enzymes β-1,3-glucanase, chitinase and protease by D. hansenii was detected in the presence of phytopathogenic fungus cell walls. The activity of β-1,3-glucanase was highest on day 12 of incubation and remained high until day 15. Chitinase and protease activities reached their highest levels on the day 15 of incubation. D. hansenii additionally demonstrated the ability to resist oxidative stress. Our data demonstrated that the main biocontrol mechanisms displayed by D. hansenii were the control of phytopathogenic fungal spore germination, production of antifungal enzymes and resistance to oxidative stress. We conclude that isolate D. hansenii UFT8422 should be further investigated for use at commercial scales at low temperatures.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1590/0001-3765202420240255
ISSN:	16782690
Additional Keywords:	Antagonistic yeasts, biological control, phytopathogen, post-harvest disease
Date made live:	02 Sep 2024 14:00 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/536402

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