Bott, Tom; Shaw, George; Gregory, Simon. 2023 A simple method for testing and controlling inhibition in soil and sediment samples for qPCR. Journal of Microbiological Methods, 212, 106795. 10.1016/j.mimet.2023.106795
The presence of polymerase-chain-reaction (PCR) inhibitors in many environmental samples can make reliable and repeatable quantitative-polymerase-chain-reaction (qPCR) analysis difficult without sample dilution. To estimate an optimal sample dilution for qPCR and reduce effects of inhibition, a simple test based on multiple dilution series of samples is presented that avoids the use of internal controls and standards reducing complexity and cost.
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