Diversity, distribution and ecology of fungal community present in Antarctic lake sediments uncovered by DNA metabarcoding

de Souza, Láuren Machado Drumond; Lirio, Juan Manuel; Coria, Silvia H.; Lopes, Fabyano Alvares Cardoso; Convey, Peter ORCID: https://orcid.org/0000-0001-8497-9903; Carvalho-Silva, Micheline; de Oliveira, Fábio Soares; Rosa, Carlos Augusto; Câmara, Paulo E.A.S.; Rosa, Luiz Henrique. 2022 Diversity, distribution and ecology of fungal community present in Antarctic lake sediments uncovered by DNA metabarcoding. Scientific Reports, 12, 8407. 8, pp. 10.1038/s41598-022-12290-6

Before downloading, please read NORA policies.

Preview

Text
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
s41598-022-12290-6.pdf - Published Version
Available under License Creative Commons Attribution 4.0.
Download (1MB) | Preview

Official URL: https://doi.org/10.1038/s41598-022-12290-6

Abstract/Summary

We assessed fungal diversity in sediments obtained from four lakes in the South Shetland Islands and James Ross Island, Antarctica, using DNA metabarcoding. We detected 218 amplicon sequence variants (ASVs) dominated by the phyla Ascomycota, Basidiomycota, Mortierellomycota, Mucoromycota and Chytridiomycota. In addition, the rare phyla Aphelidiomycota, Basidiobolomycota, Blastocladiomycota, Monoblepharomycota, Rozellomycota and Zoopagomycota as well as fungal-like Straminopila belonging to the phyla Bacillariophyta and Oomycota were detected. The fungal assemblages were dominated by unknown fungal taxa (Fungal sp. 1 and Fungal sp. 2), followed by Talaromyces rubicundus and Dactylonectria anthuriicola. In general, they displayed high diversity, richness and moderate dominance. Sequences representing saprophytic, pathogenic and symbiotic fungi were detected, including the phytopathogenic fungus D. anthuriicola that was abundant, in the relatively young Soto Lake on Deception Island. The lake sediments studied contained the DNA of rich, diverse and complex fungal communities, including both fungi commonly reported in Antarctica and other taxa considered to be rare. However, as the study was based on the use of environmental DNA, which does not unequivocally confirm the presence of active or viable organisms, further studies using other approaches such as shotgun sequencing are required to elucidate the ecology of fungi in these Antarctic lake sediments.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1038/s41598-022-12290-6
ISSN:	20452322
Additional Keywords:	Antarctica; extremophiles; freshwater; fungi; taxonomy
Date made live:	24 May 2022 07:24 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/531743

Item Type:

Publication - Article

Digital Object Identifier (DOI):

10.1038/s41598-022-12290-6

ISSN:

20452322

Additional Keywords:

Antarctica; extremophiles; freshwater; fungi; taxonomy

Date made live:

24 May 2022 07:24 +0 (UTC)

URI:

https://nora.nerc.ac.uk/id/eprint/531743