What is on the outside matters — surface charge and dissolve organic matter association affect the toxicity and physiological mode of action of polystyrene nanoplastics to C. elegans

To better understand nanoplastic effects, the potential for surface functionalization and dissolve organic matter eco-corona formation to modify the mechanisms of action and toxicity of different nanoplastics needs to be established. Here, we assess how different surface charges modifying functionalization (postive (+ve) aminated; neutral unfunctionalized; negative (−ve) carboxylated) altered the toxicity of 50 and 60 nm polystyrene nanoplastics to the nematode Caenorhabditis elegans. The potency for effects on survival, growth, and reproduction reduced in the order +ve aminated > neutral unfunctionalized ≫ −ve carboxylated with toxicity >60-fold higher for the +ve than −ve charged forms. Toxicokinetic–toxicodynamic modeling (DEBtox) showed that the charge-related potency was primarily linked to differences in effect thresholds and dose-associated damage parameters, rather than to toxicokinetic parameters. This suggests that surface functionalization may change the nature of nanoplastic interactions with membrane and organelles leading to variations in toxicity. Eco-corona formation reduced the toxicity of all nanoplastics indicating that organic molecule associations may passivate surfaces. Between particles, eco-corona interactions resulting in more equivalent effects; however, even despite these changes, the order of potency of the charged forms was retained. These results have important implications for the development of future grouping approaches.