Periphyton diversity in two different Antarctic lakes assessed using metabarcoding

Câmara, Paulo E.A.S.; de Souza, Láuren M.D.; Pinto, Otávio Henrique Bezerra; Convey, Peter ORCID: https://orcid.org/0000-0001-8497-9903; Amorim, Eduardo T.; Carvalho-Silva, Micheline; Rosa, Luiz Henrique. 2021 Periphyton diversity in two different Antarctic lakes assessed using metabarcoding. Antarctic Science, 33 (6). 596-604. https://doi.org/10.1017/S0954102021000316

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Abstract/Summary

Antarctic lakes have generally simple periphyton communities when compared with those of lower latitudes. To date, assessment of microbial diversity in Antarctica has relied heavily on traditional direct observation and cultivation methods. In this study, sterilized cotton baits were left submerged for two years in two lakes on King George Island and Deception Island, South Shetland Islands (Maritime Antarctic), followed by assessment of diversity by metabarcoding using high-throughput sequencing. DNA sequences of 44 taxa belonging to four kingdoms and seven phyla were found. Thirty-six taxa were detected in Hennequin Lake on King George Island and 20 taxa were detected in Soto Lake on Deception Island. However, no significant difference in species composition was detected between the two assemblages (Shannon index). Our data suggest that metabarcoding provides a suitable method for the assessment of periphyton biodiversity in oligotrophic Antarctic lakes.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1017/S0954102021000316
ISSN:	0954-1020
Additional Keywords:	Metabarcoding, Internal Transcribed Spacer, Deception Island, King George Island
Date made live:	24 Sep 2021 13:16 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/528900

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