Proteomics of intracellular freezing survival

Thorne, Michael A.S. ORCID: https://orcid.org/0000-0001-7759-612X; Britovšek, Nina Kočevar; Hawkins, Liam; Lilley, Kathryn S.; Storey, Kenneth. 2020 Proteomics of intracellular freezing survival. PLoS ONE, 15 (5), e0233048. 15, pp. 10.1371/journal.pone.0233048

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Abstract/Summary

Panagrolaimus sp. DAW1, a nematode cultured from the Antarctic, has the extraordinary physiological ability to survive total intracellular freezing throughout all of its compartments. While a few other organisms, all nematodes, have subsequently also been found to survive freezing in this manner, P. sp. DAW1 has so far shown the highest survival rates. In addition, P. sp. DAW1 is also, depending on the rate or extent of freezing, able to undergo cryoprotective dehydration. In this study, the proteome of P. sp DAW1 is explored, highlighting a number of differentially expressed proteins and pathways that occur when the nematodes undergo intracellular freezing. Among the strongest signals after being frozen is an upregulation of proteases and the downregulation of cytoskeletal and antioxidant activity, the latter possibly accumulated before freezing much in the way the sugar trehalose has been shown to be stored during acclimation.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1371/journal.pone.0233048
ISSN:	1932-6203
Additional Keywords:	Protein domains, Gene ontologies, Structural proteins, Trehalose, Motor proteins Protein expression Translation initiation Proteomics
Date made live:	27 May 2020 09:31 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/527823

Item Type:

Publication - Article

Digital Object Identifier (DOI):

10.1371/journal.pone.0233048

ISSN:

1932-6203

Additional Keywords:

Protein domains, Gene ontologies, Structural proteins, Trehalose, Motor proteins Protein expression Translation initiation Proteomics

Date made live:

27 May 2020 09:31 +0 (UTC)

URI:

https://nora.nerc.ac.uk/id/eprint/527823