The pH optimum of soil exoenzymes adapt to long term changes in soil pH

Puissant, Jérémy; Jones, Briony ORCID: https://orcid.org/0000-0003-4428-583X; Goodall, Tim ORCID: https://orcid.org/0000-0002-1526-4071; Mang, Dana; Blaud, Aimeric; Gweon, Hyun Soon ORCID: https://orcid.org/0000-0002-6218-6301; Malik, Ashish; Jones, Davey L.; Clark, Ian M.; Hirsch, Penny R.; Griffiths, Robert ORCID: https://orcid.org/0000-0002-3341-4547. 2019 The pH optimum of soil exoenzymes adapt to long term changes in soil pH. Soil Biology and Biochemistry, 138, 107601. 9, pp. 10.1016/j.soilbio.2019.107601

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Official URL: http://dx.doi.org/10.1016/j.soilbio.2019.107601

Abstract/Summary

Soil exoenzymes released by microorganisms break down organic matter and are crucial in regulating C, N and P cycling. Soil pH is known to influence enzyme activity, and is also a strong driver of microbial community composition; but little is known about how alterations in soil pH affect enzymatic activity and how this is mediated by microbial communities. To assess long term enzymatic adaptation to soil pH, we conducted enzyme assays at buffered pH levels on two historically managed soils maintained at either pH 5 or 7 from the Rothamsted Park Grass Long-term experiment. The pH optima for a range of exoenzymes involved in C, N, P cycling, differed between the two soils, the direction of the shift being toward the source soil pH, indicating the production of pH adapted isoenzymes by the soil microbial community. Soil bacterial and fungal communities determined by amplicon sequencing were clearly distinct between pH 5 and soil pH 7 soils, possibly explaining differences in enzymatic responses. Furthermore, β-glucosidase gene sequences extracted from metagenomes revealed an increased abundance of Acidobacterial producers in the pH 5 soils, and Actinobacteria in pH 7 soils. Our findings demonstrate that the pH optimum of soil exoenzymes adapt to long term changes in soil pH, the direction being dependent on the soil pH shift; and we provide further evidence that changes in functional microbial communities may underpin this phenomena, though new research is now needed to directly link change in enzyme activity optima with microbial communities. More generally, our new findings have large implications for modelling the efficiency of different microbial enzymatic processes under changing environmental conditions.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1016/j.soilbio.2019.107601
UKCEH and CEH Sections/Science Areas:	Soils and Land Use (Science Area 2017-) UKCEH Fellows Unaffiliated
ISSN:	0038-0717
Additional Keywords:	enzyme activity, adaptation, liming, carbon degradation, metagenomics, microbial community
NORA Subject Terms:	Agriculture and Soil Science
Date made live:	18 Nov 2019 12:17 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/525907

Item Type:

Publication - Article

Digital Object Identifier (DOI):

10.1016/j.soilbio.2019.107601

UKCEH and CEH Sections/Science Areas:

Soils and Land Use (Science Area 2017-)
UKCEH Fellows
Unaffiliated

ISSN:

0038-0717

Additional Keywords:

enzyme activity, adaptation, liming, carbon degradation, metagenomics, microbial community

NORA Subject Terms:

Agriculture and Soil Science