‘Ready Mixed’, improved nucleic acid amplification assays for the detection of Escherichia coli DNA and RNA

McQuillan, Jonathan S.; Wilson, Matthew W.. 2019 ‘Ready Mixed’, improved nucleic acid amplification assays for the detection of Escherichia coli DNA and RNA. Journal of Microbiological Methods, 165. 105721. 10.1016/j.mimet.2019.105721

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Official URL: http://dx.doi.org/10.1016/j.mimet.2019.105721

Abstract/Summary

The selective amplification of E. coli nucleic acid sequences could be used for the early warning of faecal contamination in environmental samples. Modified assays for E. coli DNA and RNA markers are presented with improved integrity and performance over existing methods, and demonstrated using ‘ready mixed’, preserved reagent mixtures.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1016/j.mimet.2019.105721
ISSN:	01677012
Date made live:	12 Nov 2019 13:59 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/525705

Item Type:

Publication - Article

Digital Object Identifier (DOI):

10.1016/j.mimet.2019.105721

ISSN:

01677012

Date made live:

12 Nov 2019 13:59 +0 (UTC)

URI:

https://nora.nerc.ac.uk/id/eprint/525705