The cryopreservation of Chlorella

Morris, G.J.; Clarke, K.J.; Clarke, Andrew ORCID: https://orcid.org/0000-0002-7582-3074. 1977 The cryopreservation of Chlorella. Archives of Microbiology, 114 (3). 249-254. 10.1007/BF00446869

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Official URL: http://dx.doi.org/10.1007/BF00446869

Abstract/Summary

Following a shift from autotrophic to heterotrophic nutrition, cells of Chlorella protothecoides become sensitive to the stresses of freezing and thawing. The injury then observed at slow rates of cooling cannot be explained by the cellular response to hypertonic solutions, and at faster cooling rates intracellular ice formation was not demonstrated to be damaging. These findings are at variance with suggested mechanisms of injury in other cellular systems. The results are compared with alterations in ultrastructure and in the composition of the cellular fatty acids.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1007/BF00446869
ISSN:	0302-8933
Date made live:	23 Oct 2019 10:29 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/525575

Item Type:

Publication - Article

Digital Object Identifier (DOI):

10.1007/BF00446869

ISSN:

0302-8933

Date made live:

23 Oct 2019 10:29 +0 (UTC)

URI:

https://nora.nerc.ac.uk/id/eprint/525575