New recombinant cold-adapted and organic solvent tolerant lipase from psychrophilic Pseudomonas sp. LSK25, isolated from Signy Island Antarctica

Salwoom, Leelatulasi; Rahman, Raja Noor Zaliha Raja Abd.; Salleh, Abu Bakar; Shariff, Fairolniza Mohd.; Convey, Peter ORCID: https://orcid.org/0000-0001-8497-9903; Ali, Mohd Shukuri Mohamad. 2019 New recombinant cold-adapted and organic solvent tolerant lipase from psychrophilic Pseudomonas sp. LSK25, isolated from Signy Island Antarctica. International Journal of Molecular Sciences, 20 (6), 1264. https://doi.org/10.3390/ijms20061264

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Abstract/Summary

In recent years, studies on psychrophilic lipases have become an emerging area of research in the field of enzymology. The study described here focuses on the cold-adapted organic solvent tolerant lipase strain Pseudomonas sp. LSK25 isolated fromSigny Station, SouthOrkney Islands,maritime Antarctic. Strain LSK25 lipase was successfully cloned, sequenced, and over-expressed in an Escherichia coli system. Sequence analysis revealed that the lipase gene of Pseudomonas sp. LSK25 consists of 1432 bp, lacks an N-terminal signal peptide and encodes a mature protein consisting of 476 amino acids. The recombinant LSK25 lipase was purified by single-step purification using Ni-Sepharose affinity chromatography and had a molecular mass of approximately 65 kDa. The final recovery and purification fold were 44% and 1.3, respectively. The LSK25 lipase was optimally active at 30 °C and at pH 6. Stable lipolytic activity was reported between temperatures of 5–30 °C and at pH 6–8. A significant enhancement of lipolytic activity was observed in the presence of Ca2+ ions, the organic lipids of rice bran oil and coconut oil, a synthetic C12 ester and a wide range of water immiscible organic solvents. Overall, lipase strain LSK25 is a potentially desirable candidate for biotechnological application, due to its stability at low temperatures, across a range of pH and in organic solvents.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.3390/ijms20061264
Additional Keywords:	Antarctica, cold adapted lipase, Pseudomonas sp. LSK25, purification, organic solvent tolerant
Date made live:	19 Mar 2019 14:40 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/521996

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.3390/ijms20061264

Additional Keywords:

Antarctica, cold adapted lipase, Pseudomonas sp. LSK25, purification, organic solvent tolerant

Date made live:

19 Mar 2019 14:40 +0 (UTC)

URI:

https://nora.nerc.ac.uk/id/eprint/521996