Growth rate and nucleic acid ratio in cultured cuttlefish Sepia officinalis (Mollusca: Cephalopoda)

Clarke, Andrew ORCID: https://orcid.org/0000-0002-7582-3074; Rodhouse, Paul G.; Holmes, Lesley J.; Pascoe, Philip L.. 1989 Growth rate and nucleic acid ratio in cultured cuttlefish Sepia officinalis (Mollusca: Cephalopoda). Journal of Experimental Marine Biology and Ecology, 133 (3). 229-240. https://doi.org/10.1016/0022-0981(89)90047-6

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Abstract/Summary

Juvenile cuttlefish Sepia offcinalis were hatched and reared in the laboratory at six ration levels and two temperatures. At 12.5 °C there was little growth whereas at 17.5 °C growth rate responded strongly to ration level. Nucleic acids were extracted into buffer containing 0.01% SDS and assayed by fluorescence using ethidium bromide and bis-benzimide and by absorbance at 260 nm. The sensitivity of nucleic acid assay using ethidium bromide was increased by shortening the excitation wavelength to 305 nm. RNA:DNA ratio was strongly correlated with growth rate in cuttlefish held at 17.5 °C; at 12.5 °C, the relationship was similar but not statistically significant. At low growth rates, RNA:DNA ratios were similar to those reported for larval fish; nucleic acid ratios at higher growth rates were higher than reported so far. This may be related to the very high growth rates achieved by many

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1016/0022-0981(89)90047-6
ISSN:	00220981
Additional Keywords:	cuttlefish, growth, RNA, DNA ratio, Sepia officinalis
Date made live:	19 Sep 2018 12:59 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/521005

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