Physiological and thylakoid ultrastructural changes in cyanobacteria in response to toxic manganese concentrations

Moura, Karen Ann Ferreira; Lizieri, Claudineia; Franco, Maione Wittig; Vaz, Marcelo Gomes Marçal Vieira; Araújo, Wagner L; Convey, Peter ORCID: https://orcid.org/0000-0001-8497-9903; Barbosa, Francisco Antônio Rodrigues. 2019 Physiological and thylakoid ultrastructural changes in cyanobacteria in response to toxic manganese concentrations. Ecotoxicology, 28 (8). 1009-1021. https://doi.org/10.1007/s10646-019-02098-y

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Official URL: https://link.springer.com/article/10.1007%2Fs10646...

Abstract/Summary

In this study, two cyanobacterial strains (morphologically identified as Microcystis novacekii BA005 and Nostoc paludosum BA033) were exposed to different Mn concentrations: 7.0, 10.5, 15.7, 23.6 and 35.4 mg L−1 for BA005; and 15.0, 22.5, 33.7, 50.6, and 76.0 mg L−1 for BA033. Manganese toxicity was assessed by growth rate inhibition (EC50), chlorophyll a content, quantification of Mn accumulation in biomass and monitoring morphological and ultrastructural effects. The Mn EC50 values were 16 mg L−1 for BA005 and 39 mg L−1 for BA033, respectively. Reduction of chlorophyll a contents and ultrastructural changes were observed in cells exposed to Mn concentrations greater than 23.6 and 33.7 mg L−1 for BA005 and BA033. Damage to intrathylakoid spaces, increased amounts of polyphosphate granules and an increased number of carboxysomes were observed in both strains. In the context of the potential application of these strains in bioremediation approaches, BA005 was able to remove Mn almost completely from aqueous medium after 96 h exposure to an initial concentration of 10.5 mg L−1, and BA033 was capable of removing 38% when exposed to initial Mn concentration of 22.5 mg L−1. Our data shed light on how these cyanobacterial strains respond to Mn stress, as well as supporting their utility as organisms for monitoring Mn toxicity in industrial wastes and potential bioremediation application.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1007/s10646-019-02098-y
ISSN:	09639292
Additional Keywords:	manganese, toxicity, cyanobacteria, ultrastructure, bioremoval
Date made live:	02 Sep 2019 11:07 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/520814

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.1007/s10646-019-02098-y

ISSN:

09639292

Additional Keywords:

manganese, toxicity, cyanobacteria, ultrastructure, bioremoval

Date made live:

02 Sep 2019 11:07 +0 (UTC)

URI:

https://nora.nerc.ac.uk/id/eprint/520814