Pseudomonas versuta sp. nov., isolated from Antarctic soil

In this study we used a polyphasic taxonomy approach to analyse three bacterial strains coded L10.10T, A4R1.5 and A4R1.12, isolated in the course of a study of quorum-quenching bacteria occurring Antarctic soil. The 16S rRNA gene sequence was identical in the three strains and showed 99.7% pairwise similarity with respect to the closest related species Pseudomonas weihenstephanensis WS4993T, and the next closest related species were P. deceptionensis M1T (99.5%), P. psychrophila E-3T, P. endophytica BSTT44T and P. fragi ATCC 4973T (99.2%). Therefore, the three strains were classified within the genus Pseudomonas. Analysis of housekeeping genes (rpoB, rpoD and gyrB) sequences showed similarities of 84-95% with respect to the closest relatives, confirming its phylogenetic affiliation. The whole genome average nucleotide identity (ANI) values were more than 99% similar among the three strains, and less than 86% to the closest related species type strains. The respiratory quinone is Q9. The major fatty acids are C16:0, C16:1 ω7c/C16:1 ω6c in summed feature 3 and C18:1 ω7c/C18:1 ω6c in summed feature 8. The strains are oxidase- and catalase-positive. The arginine dihydrolase and urease tests are positive. Growth occurs at 4–30 °C with an optimum at 28 °C, and at pH 4.0–10. The DNA G + C content is 58.2–58.3 mol %. The combined genotypic, phenotypic and chemotaxonomic data support the classification of strains L10.10T, A4R1.5 and A4R1.12 into a novel species of Pseudomonas, for which the name P. versuta sp. nov. is proposed. The type strain is L10.10T (LMG 29628T, DSM 101070T).