Evidence for polyploidy in the globally important diazotroph Trichodesmium

Sargent, Elizabeth C.; Hitchcock, Andrew; Johansson, S. Andreas; Langlois, Rebecca; Moore, C. Mark; LaRoche, Julie; Poulton, Alex J.; Bibby, Thomas S.; Bothe, Hermann. 2016 Evidence for polyploidy in the globally important diazotroph Trichodesmium. FEMS Microbiology Letters, 363 (21). fnw244. 10.1093/femsle/fnw244

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Copyright © 2016 Oxford University Press This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Geophysical Journal International following peer review. The definitive publisher-authenticated version is available online at: http://dx.doi.org/10.1093/femsle/fnw244
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Official URL: http://dx.doi.org/10.1093/femsle/fnw244

Abstract/Summary

Polyploidy is a well-described trait in some prokaryotic organisms; however, it is unusual in marine microbes from oligotrophic environments, which typically display a tendency towards genome streamlining. The biogeochemically significant diazotrophic cyanobacterium Trichodesmium is a potential exception. With a relatively large genome and a comparatively high proportion of non-protein-coding DNA, Trichodesmium appears to allocate relatively more resources to genetic material than closely related organisms and microbes within the same environment. Through simultaneous analysis of gene abundance and direct cell counts, we show for the first time that Trichodesmium spp. can also be highly polyploid, containing as many as 100 genome copies per cell in field-collected samples and >600 copies per cell in laboratory cultures. These findings have implications for the widespread use of the abundance of the nifH gene (encoding a subunit of the N2-fixing enzyme nitrogenase) as an approach for quantifying the abundance and distribution of marine diazotrophs. Moreover, polyploidy may combine with the unusual genomic characteristics of this genus both in reflecting evolutionary dynamics and influencing phenotypic plasticity and ecological resilience.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1093/femsle/fnw244
ISSN:	1574-6968
Additional Keywords:	Trichodesmium; cyanobacteria; polyploidy; nifH; nitrogenase; diazotroph
Date made live:	24 Nov 2016 14:29 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/515241

Item Type:

Publication - Article

Digital Object Identifier (DOI):

10.1093/femsle/fnw244

ISSN:

1574-6968

Additional Keywords:

Trichodesmium; cyanobacteria; polyploidy; nifH; nitrogenase; diazotroph

Date made live:

24 Nov 2016 14:29 +0 (UTC)

URI:

https://nora.nerc.ac.uk/id/eprint/515241