An Antarctic molluscan biomineralisation tool-kit

Sleight, Victoria A. ORCID: https://orcid.org/0000-0003-0550-8500; Marie, Benjamin; Jackson, Daniel J.; Dyrynda, Elizabeth A.; Marie, Arul; Clark, Melody S. ORCID: https://orcid.org/0000-0002-3442-3824. 2016 An Antarctic molluscan biomineralisation tool-kit. Scientific Reports, 6, 36978. 13, pp. https://doi.org/10.1038/srep36978

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Abstract/Summary

The Antarctic clam Laternula elliptica lives almost permanently below 0 °C and therefore is a valuable and tractable model to study the mechanisms of biomineralisation in cold water. The present study employed a multidisciplinary approach using histology, immunohistochemistry, electron microscopy, proteomics and gene expression to investigate this process. Thirty seven proteins were identified via proteomic extraction of the nacreous shell layer, including two not previously found in nacre; a novel T-rich Mucin-like protein and a Zinc-dependent metalloprotease. In situ hybridisation of seven candidate biomineralisation genes revealed discrete spatial expression patterns within the mantle tissue, hinting at modular organisation, which is also observed in the mantle tissues of other molluscs. All seven of these biomineralisation candidates displayed evidence of multifunctionality and strong association with vesicles, which are potentially involved in shell secretion in this species.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1038/srep36978
Programmes:	BAS Programmes > BAS Programmes 2015 > Biodiversity, Evolution and Adaptation
ISSN:	20452322
Additional Keywords:	environmental science, gene expression, proteomics
Date made live:	21 Nov 2016 09:42 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/513908

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