Lehmann, Katja. 2016 Sampling of riverine or marine bacterial communities in remote locations: from field to publication. In: Bourlat, Sarah J., (ed.) Marine genomics: methods and protocols. New York, Humana Press, 1-11. (Methods in Molecular Biology, 1452).
Abstract
This protocol describes how to sample and preserve microbial water column samples from rivers that can be used for 16S or 18S metabarcoding studies or shotgun sequencing. It further describes how to extract the DNA for sequencing and how to prepare raw Ilumina MiSeq amplicon data and analyze it in the R environment.
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Programmes:
CEH Science Areas 2013- > Ecological Processes & Resilience
CEH Science Areas 2013- > Environmental Informatics
CEH Science Areas 2013- > Water Resources
CEH Science Areas 2013- > Environmental Informatics
CEH Science Areas 2013- > Water Resources
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