Sampling of riverine or marine bacterial communities in remote locations: from field to publication

Lehmann, Katja. 2016 Sampling of riverine or marine bacterial communities in remote locations: from field to publication. In: Bourlat, Sarah J., (ed.) Marine genomics: methods and protocols. New York, Humana Press, 1-11. (Methods in Molecular Biology, 1452).

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Official URL: http://dx.doi.org/10.1007/978-1-4939-3774-5_1

Abstract/Summary

This protocol describes how to sample and preserve microbial water column samples from rivers that can be used for 16S or 18S metabarcoding studies or shotgun sequencing. It further describes how to extract the DNA for sequencing and how to prepare raw Ilumina MiSeq amplicon data and analyze it in the R environment.

Item Type:	Publication - Book Section
Digital Object Identifier (DOI):	10.1007/978-1-4939-3774-5_1
UKCEH and CEH Sections/Science Areas:	Acreman
ISBN:	9781493937721
Additional Keywords:	biodiversity, riverine microbial communities, community analysis, Illumina MiSeq
NORA Subject Terms:	Ecology and Environment Marine Sciences Biology and Microbiology
Date made live:	09 Mar 2016 12:13 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/512365

Item Type:

Publication - Book Section

Digital Object Identifier (DOI):

10.1007/978-1-4939-3774-5_1

UKCEH and CEH Sections/Science Areas:

Acreman

ISBN:

9781493937721

Additional Keywords:

biodiversity, riverine microbial communities, community analysis, Illumina MiSeq

NORA Subject Terms:

Ecology and Environment
Marine Sciences
Biology and Microbiology