In situ associations between marine photosynthetic picoeukaryotes and potential parasites - a role for fungi?

Lepère, Cécile; Ostrowski, Martin; Hartmann, Manuela; Zubkov, Mikhail V.; Scanlan, David J.. 2016 In situ associations between marine photosynthetic picoeukaryotes and potential parasites - a role for fungi? Environmental Microbiology Reports, 8 (4). 445-451. 10.1111/1758-2229.12339

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Preview	Text This is the peer reviewed version of the following article: Lepère, Cécile, Ostrowski, Martin, Hartmann, Manuela, Zubkov, Mikhail V. and Scanlan, David J. (2015) In situ associations between marine photosynthetic picoeukaryotes and potential parasites - a role for fungi? Environmental Microbiology Reports, which has been published in final form at doi:10.1111/1758-2229.12339. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. Lepère, Cécile, Ostrowski, Martin, Hartmann, Manuela, Zubkov, Mikhail V. and Scanlan, David J. (2015) In situ associations between marine photosynthetic picoeukaryotes and potential parasites - a role for fungi? Environmental Microbiology Reports, (doi:10.1111/1758-2229.12339). Lep-re_et_al-2015-Environmental_Microbiology_Reports-accepted.pdf - Accepted Version Download (271kB) | Preview
	Text (Suppementary information) emi4_12339_Lepere et al. SuppInfo_accepted.docx - Accepted Version Download (36kB)
	Other (Supplementary table) emi4_12339_Lepere et al. SupTable 1 accepted.xlsx - Accepted Version Download (30kB)

Abstract/Summary

Photosynthetic picoeukaryotes (PPEs) are important components of the marine picophytoplankton community playing a critical role in CO2 fixation but also as bacterivores, particularly in the oligotrophic gyres. Despite an increased interest in these organisms and an improved understanding of the genetic diversity of this group, we still know little of the environmental factors controlling the abundance of these organisms. Here, we investigated the quantitative importance of eukaryotic parasites in the free-living fraction as well as in associations with PPEs along a transect in the South Atlantic. Using tyramide signal amplification-fluorescence in situ hybridization (TSA-FISH), we provide quantitative evidence of the occurrence of free-living fungi in open ocean marine systems, while the Perkinsozoa and Syndiniales parasites were not abundant in these waters. Using flow cytometric cell sorting of different PPE populations followed by a dual-labelled TSA-FISH approach, we also demonstrate fungal associations, potentially parasitic, occurring with both pico-Prymnesiophyceae and pico-Chrysophyceae. These data highlight the necessity for further work investigating the specific role of marine fungi as parasites of phytoplankton to improve understanding of carbon flow in marine ecosystems.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1111/1758-2229.12339
ISSN:	17582229
NORA Subject Terms:	Marine Sciences
Date made live:	02 Dec 2015 10:24 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/512348

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