Seasonal variation in denitrification and dissimilatory nitrate reduction to ammonia process rates and corresponding key functional genes along an estuarine nitrate gradient

Smith, Cindy J.; Dong, Liang F.; Wilson, John; Stott, Andrew; Osborn, A. Mark; Nedwell, David B.. 2015 Seasonal variation in denitrification and dissimilatory nitrate reduction to ammonia process rates and corresponding key functional genes along an estuarine nitrate gradient. Frontiers in Microbiology, 6, 542. 11, pp. 10.3389/fmicb.2015.00542

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Abstract/Summary

This research investigated spatial-temporal variation in benthic bacterial community structure, rates of denitrification and dissimilatory nitrate reduction to ammonium (DNRA) processes and abundances of corresponding genes and transcripts at three sites—the estuary-head, mid-estuary and the estuary mouth (EM) along the nitrate gradient of the Colne estuary over an annual cycle. Denitrification rates declined down the estuary, while DNRA rates were higher at the estuary head and middle than the EM. In four out of the six 2-monthly time-points, rates of DNRA were greater than denitrification at each site. Abundance of gene markers for nitrate-reduction (nitrate reductase narG and napA), denitrification (nitrite reductase nirS) and DNRA (DNRA nitrite reductase nrfA) declined along the estuary with significant relationships between denitrification and nirS abundance, and DNRA and nrfA abundance. Spatially, rates of denitrification, DNRA and corresponding functional gene abundances decreased along the estuary. However, temporal correlations between rate processes and functional gene and transcript abundances were not observed.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.3389/fmicb.2015.00542
UKCEH and CEH Sections/Science Areas:	Shore
ISSN:	1664-302X
Additional Information. Not used in RCUK Gateway to Research.:	Open Access paper - full text available via Official URL link.
Additional Keywords:	nitrogen, nitrous oxide, denitrification / DNRA, narG, napA, nirS, nrfA, (RT)-Q-PCR
NORA Subject Terms:	Ecology and Environment
Date made live:	17 Aug 2015 13:24 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/511561

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