Key metabolic pathways involved in xenobiotic biotransformation and stress responses revealed by transcriptomics of the mangrove oyster Crassostrea brasiliana

Lüchmann, Karim H.; Clark, Melody S. ORCID: https://orcid.org/0000-0002-3442-3824; Bainy, Afonso C.D.; Gilbert, Jack A.; Craft, John A.; Chipman, J.Kevin; Thorne, Michael A.S. ORCID: https://orcid.org/0000-0001-7759-612X; Mattos, Jacó J.; Siebert, Marília N.; Schroeder, Declan C.. 2015 Key metabolic pathways involved in xenobiotic biotransformation and stress responses revealed by transcriptomics of the mangrove oyster Crassostrea brasiliana. Aquatic Toxicology, 166. 10-20. https://doi.org/10.1016/j.aquatox.2015.06.012

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This article has been accepted for publication and will appear in a revised form in Aquatic Toxicology, published by Elsevier. Copyright Elsevier.
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Official URL: http://dx.doi.org/10.1016/j.aquatox.2015.06.012

Abstract/Summary

The Brazilian oyster Crassostrea brasiliana was challenged to three common environmental contaminants: phenanthrene, diesel fuel water-accommodated fraction (WAF) and domestic sewage. Total RNA was extracted from the gill and digestive gland, and cDNA libraries were sequenced using the 454 FLX platform. The assembled transcriptome resulted in ̃20,000 contigs, which were annotated to produce the first de novo transcriptome for C. brasiliana. Sequences were screened to identify genes potentially involved in the biotransformation of xenobiotics and associated antioxidant defence mechanisms. These gene families included those of the cytochrome P450 (CYP450), 70kDa heat shock, antioxidants, such as glutathione S-transferase, superoxide dismutase, catalase and also multi-drug resistance proteins. Analysis showed that the massive expansion of the CYP450 and HSP70 family due to gene duplication identified in the Crassostrea gigas genome also occurred in C. brasiliana, suggesting these processes form the base of the Crassostrea lineage. Preliminary expression analyses revealed several candidates biomarker genes that were up-regulated during each of the three treatments, suggesting the potential for environmental monitoring.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1016/j.aquatox.2015.06.012
Programmes:	BAS Programmes > BAS Programmes 2015 > Biodiversity, Evolution and Adaptation
ISSN:	0166445X
Additional Keywords:	xenobiotic metabolism, antioxidant parameters, pollutants, bioaccumulation, bivalve, polycyclic aromatic hydrocarbon
Date made live:	14 Jul 2015 12:25 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/511312

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.1016/j.aquatox.2015.06.012

Programmes:

BAS Programmes > BAS Programmes 2015 > Biodiversity, Evolution and Adaptation

ISSN:

0166445X

Additional Keywords:

xenobiotic metabolism, antioxidant parameters, pollutants, bioaccumulation, bivalve, polycyclic aromatic hydrocarbon

Date made live:

14 Jul 2015 12:25 +0 (UTC)