Pickering, A.D.; Pottinger, T.G.. 1985 Acclimation of the brown trout, Salmo trutta L., to the stress of daily exposure to malachite green. Aquaculture, 44 (2). 145-152. 10.1016/0044-8486(85)90017-1
Abstract
Exposure of brown trout to a single dose of malachite green (2.27 ppm) caused a significant elevation of blood cortisol levels within 1 h. After 4 weeks of daily exposure to malachite green, however, this stress response was totally abolished. Daily treatment had no long-term effects on basal plasma cortisol levels, or on the numbers of circulating erythrocytes, thrombocytes and lymphocytes. Blood neutrophil numbers were significantly reduced in malachite green-treated fish, an effect thought to be due to a decreased ectoparasitic load.
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