Estrogen-binding sites in the liver of sexually mature male and female brown trout, Salmo trutta L.

Pottinger, T.G.. 1986 Estrogen-binding sites in the liver of sexually mature male and female brown trout, Salmo trutta L. General and Comparative Endocrinology, 61 (1). 120-126. 10.1016/0016-6480(86)90256-X

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Abstract/Summary

Binding sites with characteristics conforming to those of a putative estrogen receptor have been identified in the liver of sexually mature male and female brown trout. [3H]Estradiol is bound with high affinity (kD in the nM range) and limited capacity (<400 fmol mg/protein) to a proteinaceous moiety in liver cytosol. Binding is highly specific, only estradiol and estrone displace specifically bound [3H]estradiol. Cortisol, testosterone, 11-ketotestosterone, and 17α, 20β-dihydroxyprogesterone are inactive. Labeled cytosol is retained on DNA-cellulose columns, eluting with 0.1 M NaCl. The liver of a sexually mature female brown trout contains more than twice as many binding sites as that of the male (168 ± 15 fmol/mg protein cf. 69 ± 4 fmol/mg protein), and no difference in kD is observed between males and females (2.6 ± 0.2 nM and 2.9 ± 0.3 nM, respectively). Plasma from both male and female brown trout was also found to bind [3H]estradiol, but with lower affinity (kD in 10−8 M range) and higher capacity (4000 – 11,000 fmol/mg protein) and with less specificity. Estradiol, testosterone, and 11-ketotestosterone all displaced plasma-bound [3H]estradiol. It is concluded that cytosol and plasma binding of estradiol in brown trout are distinct, and indicative of the presence of an intracellular hepatic estrogen receptor and a plasma sex-steroid-binding protein.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1016/0016-6480(86)90256-X
UKCEH and CEH Sections/Science Areas:	_ Pre-2000 sections
ISSN:	0016-6480
Additional Keywords:	Brown trout, liver, vitellogenesis, estradiol receptor, ER, sex hormone binding globulin, SHBG, endocrine disruption
NORA Subject Terms:	Zoology Biology and Microbiology
Date made live:	21 Sep 2015 13:18 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/511188

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