ACTH does not mediate divergent stress responsiveness in rainbow trout

Pottinger, Tom G.; Carrick, Toby R.. 2001 ACTH does not mediate divergent stress responsiveness in rainbow trout. Comparative Biochemistry and Physiology Part A, 129 (2-3). 399-404. 10.1016/S1095-6433(00)00357-3

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Abstract/Summary

Two lines of rainbow trout selected for high (HR) and low (LR) responsiveness to a standardised confinement stressor displayed a sustained divergence in plasma cortisol levels during a 3 h period of confinement (max.: HR: 167 ± 13 ng ml-1; LR: 103 ± 8 ng ml-1; P < 0.001). However, no significant difference in plasma ACTH levels was evident (max: HR: 153 ± 9 pg ml-1; LR: 142 ± 7 pg ml-1). Dexamethasone (DEX) was administered to HR and LR fish to block endogenous adrenocorticotropin (ACTH) release. Administration of a weight-adjusted dose of ACTH to the DEX-blocked fish elevated plasma cortisol levels to a significantly greater extent in HR (233 ± 24 ng ml-1) than LR (122 ± 14 ng ml-1) fish (P < 0.001). Plasma cortisol levels in DEX-blocked HR and LR fish after sham injection were low but also significantly different (HR: 6.7 ± 1 ng ml-1; LR: 2.2 ± 0.2 ng ml-1; P < 0.001). These results indicate that modulation of cortisol responsiveness to stressors in HR and LR fish resides, at least in part, downstream of the hypothalamic-pituitary axis.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1016/S1095-6433(00)00357-3
Programmes:	CEH Programmes pre-2009 publications > Other
UKCEH and CEH Sections/Science Areas:	_ Environmental Chemistry & Pollution
ISSN:	1095-6433
Additional Information. Not used in RCUK Gateway to Research.:	NOTICE: this is the author’s version of a work that was accepted for publication in Soil Biology and Biochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Comparative Biochemistry and Physiology Part A 129 2001 399-404. doi:10.1016/S1095-6433(00)00357-3 www.elsevier.com/
Additional Keywords:	stress, rainbow trout, Oncorhynchus mykiss, cortisol, ACTH, dexamethasone, selective breeding, coping strategies
NORA Subject Terms:	Zoology Biology and Microbiology
Date made live:	30 Jun 2015 15:10 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/510818

Item Type:

Publication - Article

Digital Object Identifier (DOI):

10.1016/S1095-6433(00)00357-3

Programmes:

CEH Programmes pre-2009 publications > Other

UKCEH and CEH Sections/Science Areas:

_ Environmental Chemistry & Pollution

ISSN:

1095-6433

Additional Information. Not used in RCUK Gateway to Research.:

NOTICE: this is the author’s version of a work that was accepted for publication in Soil Biology and Biochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Comparative Biochemistry and Physiology Part A 129 2001 399-404. doi:10.1016/S1095-6433(00)00357-3 www.elsevier.com/

Additional Keywords:

stress, rainbow trout, Oncorhynchus mykiss, cortisol, ACTH, dexamethasone, selective breeding, coping strategies

NORA Subject Terms:

Zoology
Biology and Microbiology

Date made live:

30 Jun 2015 15:10 +0 (UTC)

URI:

https://nora.nerc.ac.uk/id/eprint/510818