Response of the ammonia oxidation activity of microorganisms in surface sediment to a controlled sub-seabed release of CO2

Watanabe, Yuji; Tait, Karen; Gregory, Simon; Hayashi, Masatoshi; Shimamoto, Akifumi; Taylor, Peter; Stahl, Henrik; Green, Kay; Yoshinaga, Ikuo; Suwa, Yuichi; Kita, Jun. 2015 Response of the ammonia oxidation activity of microorganisms in surface sediment to a controlled sub-seabed release of CO2. International Journal of Greenhouse Gas Control, 38. 162-170. 10.1016/j.ijggc.2014.11.013

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Official URL: http://dx.doi.org/10.1016/j.ijggc.2014.11.013

Abstract/Summary

The impact of a sub-seabed CO2 leak from geological sequestration on the microbial process of ammonia oxidation was investigated in the field. Sediment samples were taken before, during and after a controlled sub-seabed CO2 leak at four zones differing in proximity to the CO2 source (epicentre, and 25 m, 75 m, and 450 m distant). The impact of CO2 release on benthic microbial ATP levels was compared to ammonia oxidation rates and the abundance of bacterial and archaeal ammonia amoA genes and transcripts, and also to the abundance of nitrite oxidizer (nirS) and anammox hydrazine oxidoreductase (hzo) genes and transcripts. The major factor influencing measurements was seasonal: only minor differences were detected at the zones impacted by CO2 (epicentre and 25 m distant). This included a small increase to ammonia oxidation after 37 days of CO2 release which was linked to an increase in ammonia availability as a result of mineral dissolution. A CO2 leak on the scale used within this study (<1 tonne day−1) would have very little impact to ammonia oxidation within coastal sediments. However, seawater containing 5% CO2 did reduce rates of ammonia oxidation. This was linked to the buffering capacity of the sediment, suggesting that the impact of a sub-seabed leak of stored CO2 on ammonia oxidation would be dependent on both the scale of the CO2 release and sediment type.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1016/j.ijggc.2014.11.013
ISSN:	17505836
Date made live:	13 Feb 2015 13:12 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/509718

Item Type:

Publication - Article

Digital Object Identifier (DOI):

10.1016/j.ijggc.2014.11.013

ISSN:

17505836

Date made live:

13 Feb 2015 13:12 +0 (UTC)

URI:

https://nora.nerc.ac.uk/id/eprint/509718