Production of extracellular hydrolase enzymes by fungi from King George Island

Krishnan, Abiramy; Convey, Peter ORCID: https://orcid.org/0000-0001-8497-9903; Gonzales-Rocha, Gerardo; Alias, Sita Aisyah. 2016 Production of extracellular hydrolase enzymes by fungi from King George Island. Polar Biology, 39 (1). 65-76. https://doi.org/10.1007/s00300-014-1606-7

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Abstract/Summary

Fungi are known to produce a range of extracellular enzymes and other secondary metabolites. Investment in extracellular enzyme production may be an important element of the survival strategy of these fungi in maritime Antarctic soils. This study focuses on fungi that were isolated from ornithogenic, undisturbed and human-impacted soils collected from the Fildes Peninsula, King George Island, Antarctica, during the austral summer in February 2007. We (1) describe fungal diversity based on molecular approaches, (2) describe the thermal characteristics of the fungal isolates, and (3) screen extracellular hydrolase enzyme production (amylase and cellulase) by the isolates. Soil samples were cultured using the Warcup soil plating technique and incubated at 4 and 25 °C to allow basic thermal classification. In total, 101 isolates were obtained. All the isolates were screened at culture temperatures of 4 and 25 °C in order to detect activity of extracellular hydrolase enzymes. At 25 °C, ornithogenic penguin rookery soils recorded the lowest diversity of fungi, with little difference in diversity apparent between the other soils examined. At 4 °C, an undisturbed site recorded the lowest and a human-impacted site the highest diversity of fungi. The majority of the fungi identified in this study were in the mesophilic thermal class. Six strains possessed significant activity for amylase and 13 for cellulase at 25 °C. At 4 °C, four strains showed significant amylase and 22 significant cellulase activity. The data presented increase our understanding of microbial responses to environmental temperature

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1007/s00300-014-1606-7
Programmes:	BAS Programmes > BAS Programmes 2015 > Biodiversity, Evolution and Adaptation
ISSN:	0722-4060
Additional Keywords:	extracellular enzymes, secondary metabolites, psychrophilic, psychrotolerant, mesophilic, screening
Date made live:	01 Dec 2014 09:32 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/504793

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.1007/s00300-014-1606-7

Programmes:

BAS Programmes > BAS Programmes 2015 > Biodiversity, Evolution and Adaptation

ISSN:

0722-4060

Additional Keywords:

extracellular enzymes, secondary metabolites, psychrophilic, psychrotolerant, mesophilic, screening

Date made live:

01 Dec 2014 09:32 +0 (UTC)