Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic peninsula: a pyrosequencing approach

Kleinteich, J; Hildebrand, F; Wood, SA; Cirés, S; Agha, R; Quesada, A; Pearce, D ORCID: https://orcid.org/0000-0001-5292-4596; Convey, P ORCID: https://orcid.org/0000-0001-8497-9903; Kupper, FC; Dietrich, DR. 2014 Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic peninsula: a pyrosequencing approach. Antarctic Science, 26 (5). 521-532. 10.1017/S0954102014000145

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Abstract/Summary

Despite their pivotal role as primary producers, there is little information as to the diversity and physiology of cyanobacteria in the meltwater ecosystems of polar regions. Thirty cyanobacterial mats from Adelaide Island, Antarctica were investigated using 16S rRNA gene pyrosequencing and automated ribosomal intergenic spacer analysis, and screened for cyanobacterial toxins using molecular and chemical approaches. A total of 274 operational taxonomic units (OTUs) were detected. The richness ranged between 8 and 33 cyanobacterial OTUs per sample, reflecting a high mat diversity. Leptolyngbya and Phormidium (c. 55% and 37% of the OTUs per mat) were dominant. Cyanobacterial community composition was similar between mats, particularly those obtained from closely adjacent locations. The cyanotoxin microcystin was detected in 26 of 27 mats (10–300 ng g-1 organic mass), while cylindrospermopsin, detected for the first time in Antarctica, was present in 21 of 30 mats (2–156 ng g-1 organic mass). The latter was confirmed via liquid chromatography-mass spectrometry and by the presence of the cyrAB and cyrJ genes. This study demonstrates the usefulness of pyrosequencing for characterizing diverse cyanobacterial communities, and confirms that cyanobacteria from extreme environments produce a similar range of cyanotoxins as their temperate counterparts.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1017/S0954102014000145
Programmes:	BAS Programmes > Polar Science for Planet Earth (2009 - ) > Ecosystems
ISSN:	0954-1020
Additional Keywords:	automated ribosomal intergenic spacer analysis, cylindrospermopsin, liquid chromatography-mass spectrometry, microcystin
Date made live:	30 May 2014 13:26 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/503228

Item Type:

Publication - Article

Digital Object Identifier (DOI):

10.1017/S0954102014000145

Programmes:

BAS Programmes > Polar Science for Planet Earth (2009 - ) > Ecosystems

ISSN:

0954-1020

Additional Keywords:

automated ribosomal intergenic spacer analysis, cylindrospermopsin, liquid chromatography-mass spectrometry, microcystin

Date made live:

30 May 2014 13:26 +0 (UTC)