Changes in seasonal expression patterns of ecdysone receptor, retinoid X receptor and an A-type allatostatin in the copepod, Calanus finmarchicus, in a sea loch environment: An investigation of possible mediators of diapause

Clark, Katie A.J.; Brierley, Andrew S.; Pond, David W.; Smith, Valerie J.. 2013 Changes in seasonal expression patterns of ecdysone receptor, retinoid X receptor and an A-type allatostatin in the copepod, Calanus finmarchicus, in a sea loch environment: An investigation of possible mediators of diapause. General and Comparative Endocrinology, 189. 66-73. 10.1016/j.ygcen.2013.04.002

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Official URL: http://dx.doi.org/10.1016/j.ygcen.2013.04.002

Abstract/Summary

The marine copepod, Calanus finmarchicus, is a crucial component of the pelagic food web in the North Atlantic and peripheral seas where it is a major player in biogeochemical cycles and the productivity of commercially important fisheries. A key stage in its life cycle is the emergence of the pre-adult, copepodite developmental stage five (CV) from a period of overwintering dormancy, known as diapause. As is the case in many insect species, diapause is also likely to be under endocrine control in C. finmarchicus. To investigate the hormonal regulation of diapause behaviour of stage CV C. finmarchicus, the expression of three key genes: ecdysone receptor (EcR), retinoid X receptor (RXR) and an A-type allatostatin (A-type AST), were measured in specimens collected at monthly intervals from Loch Etive, a ca. 150 m deep sea loch on the west coast of Scotland, between June 2006 and May 2007. The full length RXR gene was cloned and sequenced from C. finmarchicus, and was found to share 49–53% total identity with equivalent genes encoding proteins from other crustaceans, and >80% identity in the DNA binding domain with other crustaceans, insects and vertebrates. EcR expression was least in December when the animals are expected to be in diapause, but began to increase in January, when the animals were terminating diapause. Concomittant with the rise in EcR in January was low expression of A-type AST and high expression of RXR.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1016/j.ygcen.2013.04.002
Programmes:	BAS Programmes > Polar Science for Planet Earth (2009 - ) > Ecosystems
ISSN:	00166480
Additional Keywords:	Diapause; Calanus finmarchicus; Gene expression; Retinoid X receptor; Ecdysone receptor; A-type allatostatin
Date made live:	14 Jun 2013 12:30 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/502244

Item Type:

Publication - Article

Digital Object Identifier (DOI):

10.1016/j.ygcen.2013.04.002

Programmes:

BAS Programmes > Polar Science for Planet Earth (2009 - ) > Ecosystems

ISSN:

00166480

Additional Keywords:

Diapause; Calanus finmarchicus; Gene expression; Retinoid X receptor; Ecdysone receptor; A-type allatostatin

Date made live:

14 Jun 2013 12:30 +0 (UTC)