Lack of an HSP70 heat shock response in two Antarctic marine invertebrates

Clark, Melody S. ORCID: https://orcid.org/0000-0002-3442-3824; Fraser, Keiron P.P. ORCID: https://orcid.org/0000-0001-5491-8376; Peck, Lloyd S. ORCID: https://orcid.org/0000-0003-3479-6791. 2008 Lack of an HSP70 heat shock response in two Antarctic marine invertebrates. Polar Biology, 31 (9). 1059-1065. https://doi.org/10.1007/s00300-008-0447-7

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Official URL: http://dx.doi.org/10.1007/s00300-008-0447-7

Abstract/Summary

Members of the HSP70 gene family comprising the inducible (HSP70) genes and GRP78 (glucose-regulated protein 78 kDa) were identified in an Antarctic sea star (Odontaster validus) and an Antarctic gammarid (Paraceradocus gibber). These genes were surveyed for expression levels via Q-PCR after an acute 2-hour heat shock experiment in both animals and a time course assay in O. validus. No significant up-regulation was detected for any of the genes in either of the animals during the acute heat shock. The time course experiment in O. validus produced slightly different results with an initial down regulation in these genes at 2°C, but no significant up-regulation of the genes either at 2 or 6°C. Therefore, the classical heat shock response is absent in both species. The data is discussed in the context of the organisms’ thermal tolerance and the applicability of HSP70 to monitor thermal stress in Antarctic marine organisms.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1007/s00300-008-0447-7
Programmes:	BAS Programmes > Global Science in the Antarctic Context (2005-2009) > Biodiversity, Functions, Limits and Adaptation from Molecules to Ecosystems
ISSN:	0722-4060
Additional Keywords:	Antarctic, Climate change, biomarker, stress, heat shock proteins
NORA Subject Terms:	Zoology Biology and Microbiology Ecology and Environment
Date made live:	29 Aug 2008 13:34 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/3951

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.1007/s00300-008-0447-7

Programmes:

BAS Programmes > Global Science in the Antarctic Context (2005-2009) > Biodiversity, Functions, Limits and Adaptation from Molecules to Ecosystems

ISSN:

0722-4060

Additional Keywords:

Antarctic, Climate change, biomarker, stress, heat shock proteins

NORA Subject Terms:

Zoology
Biology and Microbiology
Ecology and Environment