Reproducibility of a microbial river water community to self-organize upon perturbation with the natural chemical enantiomers, R- and S-carvone

Lehmann, Katja; Crombie, Andrew; Singer, Andrew C. ORCID: https://orcid.org/0000-0003-4705-6063. 2008 Reproducibility of a microbial river water community to self-organize upon perturbation with the natural chemical enantiomers, R- and S-carvone. FEMS Microbiology Ecology, 66 (2). 208-220.

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Abstract/Summary

A river water microbial community was studied in response to perturbation with the monoterpene enantiomers R- and S-carvone. The microbial community structure and function was also evaluated after enantiomers exposure was switched. Microbial communities were evaluated by length heterogeneity PCR. The addition of R- and S-carvone enriched for a range of functionally different communities: : enantiomer-selective, racemic and ones that contain both. After 5 days incubation, the R- and S-carvone treatments developed a range of dominant microbial communities, which were increasingly dissimilar from the ones in which no carvone degradation had taken part (R-values: R-carvone 0.743, S-carvone 0.5007 ).Upon carvone depletion, communities reverted to a less dominant community structure. After the cross-over, the rate of carvone utilization was significantly faster than after the first carvone addition (p=0.008) as demonstrated by concomitant carvone and oxygen depletion. The main R-degrading community (450 to 456 bp) appeared enantioselective and largely unable to degrade S-carvone, whereas the S-carvone degrading community (502 to 508 bp) appeared to have racemic catabolic capacity. In conclusion, chemical perturbations, such as enantiomers, might generate a significant shift in the river microbial ecology that can have implications for the function of a river in both a spatial and temporal context.

Item Type:	Publication - Article
Programmes:	CEH Programmes pre-2009 publications > Biogeochemistry > SE01B Sustainable Monitoring, Risk Assessment and Management of Chemicals CEH Programmes pre-2009 publications > Biodiversity > BD03 The Genetic Basis of Ecological Function
UKCEH and CEH Sections/Science Areas:	Hails
ISSN:	0168-6496
Additional Keywords:	river water, self organization, microbiology, microbial ecology, length heterogeneity PCR, carvone, biodegradation
NORA Subject Terms:	Biology and Microbiology Ecology and Environment
Date made live:	04 Nov 2008 14:55 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/3589

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