Simultaneous high speed optical and impedance analysis of single particles with a microfluidic cytometer

Barat, David; Spencer, Daniel; Benazzi, Giuseppe; Mowlem, Matthew Charles; Morgan, Hywel. 2012 Simultaneous high speed optical and impedance analysis of single particles with a microfluidic cytometer. Lab on a Chip, 12 (1). 118-126. 10.1039/c1lc20785g

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Abstract/Summary

We describe a microfluidic cytometer that performs simultaneous optical and electrical characterisation of particles. The microfluidic chip measures side scattered light, signal extinction and fluorescence using integrated optical fibres coupled to photomultiplier tubes. The channel is 80 mm high and 200 mm wide, and made from SU-8 patterned and sandwiched between glass substrates. Particles were focused into the analysis region using 1-D hydrodynamic focusing and typical particle velocities were 0.1 ms�1. Excitation light is coupled into the detection channel with an optical fibre and focused into the channel using an integrated compound air lens. The electrical impedance of particles is measured at 1 MHz using micro-electrodes fabricated on the channel top and bottom. This data is used to accurately size the particles. The system is characterised using a range of different sized polystyrene beads (fluorescent and non-fluorescent). Single and mixed populations of beads were measured and the data compared with a conventional flow cytometer.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1039/c1lc20785g
Programmes:	NOC Programmes
ISSN:	14730197
Date made live:	10 Jan 2012 10:47 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/307499

Item Type:

Publication - Article

Digital Object Identifier (DOI):

10.1039/c1lc20785g

Programmes:

NOC Programmes

ISSN:

14730197

Date made live:

10 Jan 2012 10:47 +0 (UTC)

URI:

https://nora.nerc.ac.uk/id/eprint/307499