Development of microsatellite markers using 454 sequencing for the rare socially parasitic hoverfly, Microdon mutabilis

von Zum Hof, Juergen; Schonrogge, Karsten; Cook, James M.; Gardner, Michael G.

NORA

Development of microsatellite markers using 454 sequencing for the rare socially parasitic hoverfly, Microdon mutabilis

von Zum Hof, Juergen; Schonrogge, Karsten ORCID: https://orcid.org/0000-0003-0122-6493; Cook, James M.; Gardner, Michael G.. 2012 Development of microsatellite markers using 454 sequencing for the rare socially parasitic hoverfly, Microdon mutabilis. Australian Journal of Zoology, 60 (2). 108-110. 10.1071/ZO12042

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Abstract

To date there have been only limited fine-scale investigations into the molecular ecology of the European hoverfly, Microdon mutabilis, due to the paucity of available polymorphic markers.Wedescribe the development of primers amplifying five novel microsatellite loci using next-generation sequencing (454) and three previously undescribed M. mutabilis microsatellite loci using enrichments. In hoverflies from a population in Ireland, the number of alleles per locus ranged from 2 to 16, and the observed heterozygosity ranged between 0.26 and 0.97.

Documents

19884:73496

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19884:73496

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Information

Programmes:

UNSPECIFIED

Official URL:

http://dx.doi.org/10.1071/ZO12042

Digital Object Identifier (DOI):

10.1071/ZO12042

Programmes (Superseded):

CEH Topics & Objectives 2009 - 2012 > Biodiversity > BD Topic 2 - Ecological Processes in the Environment > BD - 2.3 - Identify indicators and traits of ecosystem status ...

UKCEH and CEH Sections/Science Areas:

Hails

ISSN:

0004-959X

NORA Subject Terms:

Ecology and Environment

Additional Keywords:

genetic population structure, local adaptation, PCR, social parasite

Additional Information:

This document is the author’s final manuscript version of the journal article, incorporating any revisions agreed during the peer review process. Some differences between this and the publisher’s version remain. You are advised to consult the publisher’s version if you wish to cite from this article. The definitive version is available at www.publish.csiro.au/

Library

URI:

https://nora.nerc.ac.uk/id/eprint/19884

Item Type:

Publication - Article

Date made live:

10 Oct 2012 13:53

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