Can genetic bar-coding be used to identify aquatic Ranunculus L. subgenus Batrachium (DC) A. Gray? A test using some species from the British Isles

Telford, Annika ORCID: https://orcid.org/0000-0002-7889-7597; O'Hare, Matthew T.; Cavers, Stephen ORCID: https://orcid.org/0000-0003-2139-9236; Holmes, Nigel. 2011 Can genetic bar-coding be used to identify aquatic Ranunculus L. subgenus Batrachium (DC) A. Gray? A test using some species from the British Isles. Aquatic Botany, 95 (1). 65-70. 10.1016/j.aquabot.2011.03.004

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Official URL: http://dx.doi.org/10.1016/j.aquabot.2011.03.004

Abstract/Summary

Aquatic Batrachium Ranunculus species are a key component of river macrophyte communities selected for protection under European Union legislation. The group’s simplified morphology and variable taxonomic interpretation often makes identification to species level very difficult. A genetic approach was trialled as an alternative, more reliable, means of identification. DNA barcoding using four markers (chloroplast and nuclear) was tested. The chloroplast sequence trnH-psbA worked best and allowed identification of three out of five species while nuclear sequences supported the identification of two hybrids. This method is amenable to simplification through techniques such as PCR-RFLP or RT-PCR. It has the potential to provide easy, rapid and inexpensive identification of Batrachium Ranunculus species.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1016/j.aquabot.2011.03.004
Programmes:	CEH Topics & Objectives 2009 - 2012 > Biodiversity > BD Topic 2 - Ecological Processes in the Environment > BD - 2.3 - Identify indicators and traits of ecosystem status ...
UKCEH and CEH Sections/Science Areas:	Watt
ISSN:	0304-3770
Additional Keywords:	Ranunculus, Batrachium,DNA barcoding, hybrid, Internal transcribed spacer(ITS), matK, trnC-ycf6, trnH-psbA
NORA Subject Terms:	Biology and Microbiology Ecology and Environment
Date made live:	25 May 2011 08:55 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/11286

Item Type:

Publication - Article

Digital Object Identifier (DOI):

10.1016/j.aquabot.2011.03.004

Programmes:

CEH Topics & Objectives 2009 - 2012 > Biodiversity > BD Topic 2 - Ecological Processes in the Environment > BD - 2.3 - Identify indicators and traits of ecosystem status ...

UKCEH and CEH Sections/Science Areas:

Watt

ISSN:

0304-3770

Additional Keywords:

Ranunculus, Batrachium,DNA barcoding, hybrid, Internal transcribed spacer(ITS), matK, trnC-ycf6, trnH-psbA