Sebacinales are associates of the leafy liverwort Lophozia excisa in the southern maritime Antarctic

Newsham, Kevin K. ORCID: https://orcid.org/0000-0002-9108-0936; Bridge, Paul D.. 2010 Sebacinales are associates of the leafy liverwort Lophozia excisa in the southern maritime Antarctic. Mycorrhiza, 20 (5). 307-313. 10.1007/s00572-009-0283-9

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Abstract/Summary

The leafy liverwort Lophozia excisa, which is colonised by basidiomycete fungi in other biomes and which evidence suggests may be colonised by mycorrhizal fungi in Antarctica, was sampled from L,onie Island in the southern maritime Antarctic (67A degrees 36' S, 68A degrees 21' W). Microscopic examination of plants indicated that fungal hyphae colonised 78% of the rhizoids of the liverwort, apparently by entering the tips of rhizoids prior to growing into their bases, where they formed hyphal coils. Extensive colonisation of stem medullary cells by hyphae was also observed. DNA was extracted from surface-sterilised liverwort tissues and sequenced following nested PCR, using the primer set ITS1F/TW14, followed by a second round of amplification using the ITSSeb3/TW13 primer set. Neighbour-joining analyses showed that the sequences obtained nested in Sebacinales clade B as a 100% supported sister group to Sebacinales sequences from the leafy liverworts Lophozia sudetica, L. incisa and Calypogeia muelleriana sampled from Europe. Direct PCR using the fungal specific primer set ITS1F/ITS4 similarly identified fungi belonging to Sebacinales clade B as the principal colonists of L. excisa tissues. These observations indicate the presence of a second mycothallus in Antarctica and support the previous suggestion that the Sebacinales has a wide geographical distribution.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	10.1007/s00572-009-0283-9
Programmes:	BAS Programmes > Polar Science for Planet Earth (2009 - ) > Ecosystems
ISSN:	0940-6360
NORA Subject Terms:	Biology and Microbiology Ecology and Environment
Date made live:	20 Aug 2010 12:31 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/10607

Item Type:

Publication - Article

Digital Object Identifier (DOI):

10.1007/s00572-009-0283-9

Programmes:

BAS Programmes > Polar Science for Planet Earth (2009 - ) > Ecosystems

ISSN:

0940-6360

NORA Subject Terms:

Biology and Microbiology
Ecology and Environment

Date made live:

20 Aug 2010 12:31 +0 (UTC)