A simple method for testing and controlling inhibition in soil and sediment samples for qPCR

Bott, Tom; Shaw, George; Gregory, Simon. 2023 A simple method for testing and controlling inhibition in soil and sediment samples for qPCR. Journal of Microbiological Methods, 212, 106795. https://doi.org/10.1016/j.mimet.2023.106795

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Official URL: http://dx.doi.org/10.1016/j.mimet.2023.106795

Abstract/Summary

The presence of polymerase-chain-reaction (PCR) inhibitors in many environmental samples can make reliable and repeatable quantitative-polymerase-chain-reaction (qPCR) analysis difficult without sample dilution. To estimate an optimal sample dilution for qPCR and reduce effects of inhibition, a simple test based on multiple dilution series of samples is presented that avoids the use of internal controls and standards reducing complexity and cost.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1016/j.mimet.2023.106795
ISSN:	01677012
Date made live:	20 Sep 2023 15:29 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/535864

Item Type:

Publication - Article

Digital Object Identifier (DOI):

https://doi.org/10.1016/j.mimet.2023.106795

ISSN:

01677012

Date made live:

20 Sep 2023 15:29 +0 (UTC)

URI:

https://nora.nerc.ac.uk/id/eprint/535864