High genetic diversity at the extreme range edge: nucleotide variation at nuclear loci in Scots pine (Pinus sylvestris L.) in Scotland

Wachowiak, W.; Salmela, M.J.; Ennos, R.A.; Iason, G.; Cavers, S. ORCID: https://orcid.org/0000-0003-2139-9236. 2011 High genetic diversity at the extreme range edge: nucleotide variation at nuclear loci in Scots pine (Pinus sylvestris L.) in Scotland. Heredity, 106. 775-787. https://doi.org/10.1038/hdy.2010.118

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Abstract/Summary

Nucleotide polymorphism at 12 nuclear loci was studied in Scots pine populations across an environmental gradient in Scotland, to evaluate the impacts of demographic history and selection on genetic diversity. At eight loci, diversity patterns were compared between Scottish and continental European populations. At these loci, a similar level of diversity (θsil=~0.01) was found in Scottish vs mainland European populations, contrary to expectations for recent colonization, however, less rapid decay of linkage disequilibrium was observed in the former (ρ=0.0086±0.0009, ρ=0.0245±0.0022, respectively). Scottish populations also showed a deficit of rare nucleotide variants (multi-locus Tajima's D=0.316 vs D=−0.379) and differed significantly from mainland populations in allelic frequency and/or haplotype structure at several loci. Within Scotland, western populations showed slightly reduced nucleotide diversity (πtot=0.0068) compared with those from the south and east (0.0079 and 0.0083, respectively) and about three times higher recombination to diversity ratio (ρ/θ=0.71 vs 0.15 and 0.18, respectively). By comparison with results from coalescent simulations, the observed allelic frequency spectrum in the western populations was compatible with a relatively recent bottleneck (0.00175 × 4Ne generations) that reduced the population to about 2% of the present size. However, heterogeneity in the allelic frequency distribution among geographical regions in Scotland suggests that subsequent admixture of populations with different demographic histories may also have played a role.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1038/hdy.2010.118
Programmes:	CEH Topics & Objectives 2009 - 2012 > Biodiversity > BD Topic 2 - Ecological Processes in the Environment > BD - 2.4 - Estimate the impact of the main drivers and pressures on biodiversity ... CEH Topics & Objectives 2009 - 2012 > Biodiversity > BD Topic 3 - Managing Biodiversity and Ecosystem Services in a Changing Environment > BD - 3.4 - Provide science-based advice ...
UKCEH and CEH Sections/Science Areas:	Watt
ISSN:	0018-067X
Additional Keywords:	Pinus sylvestris, candidate gene, adaptation, bottleneck, nucleotide diversity, population differentiation, linkage disequilibrium, recolonization
NORA Subject Terms:	Ecology and Environment
Related URLs:	Author's Homepage
Date made live:	17 May 2011 15:14 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/10957

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