Effects of active silicon uptake by rice on 29Si fractionation in various plant parts

Köster, J.R.; Bol, R.; Leng, Melanie ORCID:; Parker, A.G.; Sloane, H.J.; Ma, J.F.. 2009 Effects of active silicon uptake by rice on 29Si fractionation in various plant parts. Rapid Communications in Mass Spectrometry, 23 (16). 2398-2402.

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Rice (Oryza sativa L.) accumulates large amounts of silicon which improves its growth and health due to enhanced resistance to biotic and abiotic stresses. Silicon uptake and loading to xylem in rice are predominantly active processes performed by transporters encoded by the recently identified genes Lsi1 (Si influx transporter gene) and Lsi2 (Si efflux transporter gene). Silicon deposition in rice during translocation to upper plant tissues is known to discriminate against the heavier isotopes 29Si and 30Si, resulting in isotope fractionation within the plant. We analyzed straw and husk samples of rice mutants defective in Lsi1, Lsi2 or both for silicon content and 29Si using isotope ratio mass spectrometry (IRMS) and compared these results with those for the corresponding wild-type varieties (WT). The silicon content was higher in husk than in straw. All the mutant rice lines showed clearly lower silicon content than the WT lines (4-23% Si of WT). The 29Si was lower in straw and husk for the uptake defective mutant (lsi1) than for WT, albeit 29Si was 0.3 higher in husk than in straw in both lines. The effect of defective efflux (lsi2) differed for straw and husk with higher 29Si in straw, but lower 29Si in husk while WT showed similar 29Si in both fractions. These initial results show the potential of Si isotopes to enlighten the influence of active uptake on translocation and deposition processes in the plant.

Item Type: Publication - Article
Digital Object Identifier (DOI):
Programmes: BGS Programmes 2009 > NERC Isotope Geoscience Laboratory
ISSN: 0951-4198
NORA Subject Terms: Botany
Ecology and Environment
Date made live: 13 Aug 2009 15:27 +0 (UTC)

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