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Assessing binary mixture effects from genotoxic and endocrine disrupting environmental contaminants using infrared spectroscopy

Morais, Camilo L.M.; Shore, Richard F.; Pereira, M. Gloria; Martin, Francis L.. 2018 Assessing binary mixture effects from genotoxic and endocrine disrupting environmental contaminants using infrared spectroscopy. ACS Omega, 3 (10). 13399-13412. https://doi.org/10.1021/acsomega.8b01916

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Abstract/Summary

Benzo[a]pyrene (B[a]P), polychlorinated biphenyls (PCBs), and polybrominated diphenyl ethers (PBDEs) are persistent contaminants and concern has arisen over co-exposure of organisms when the chemicals exist in mixtures. Herein, attenuated total reflection Fourier transform infrared spectroscopy was used to identify biochemical alterations induced in cells by single and binary mixtures of these environmental chemicals. It was also investigated as a method to identify if interactions are occurring in mixtures and as a possible tool to predict mixture effects. Mallard fibroblasts were treated with single and binary mixtures of B[a]P, PCB126, PCB153, BDE47, and BDE209. Comparison of observed spectra from cells treated with binary mixtures with expected additive spectra, which were created from individual exposure spectra, indicated that in many areas of the spectrum, less-than-additive binary mixture effects may occur. However, possible greater-than-additive alterations were identified in the 1650–1750 cm–1 lipid region and may demonstrate a common mechanism of B[a]P and PCBs or PBDEs, which can enhance toxicity in mixtures.

Item Type: Publication - Article
Digital Object Identifier (DOI): https://doi.org/10.1021/acsomega.8b01916
UKCEH and CEH Sections/Science Areas: Pollution (Science Area 2017-)
ISSN: 2470-1343
Additional Information. Not used in RCUK Gateway to Research.: Open Access paper - full text available via Official URL link.
NORA Subject Terms: Zoology
Date made live: 02 Nov 2018 12:09 +0 (UTC)
URI: http://nora.nerc.ac.uk/id/eprint/521424

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