Species-specific influence of Pi-status on inorganic carbon acquisition in microalgae (Chlorophyceae)

Lachmann, Sabrina C.; Maberly, Stephen C.; Spijkerman, Elly. 2017 Species-specific influence of Pi-status on inorganic carbon acquisition in microalgae (Chlorophyceae). Botany, 95 (9). 943-952.

Before downloading, please read NORA policies.
[img] Text
Specific_Pi_Bot_Rev_final.docx - Accepted Version
Restricted to NORA staff only

Download (74kB)


Inorganic phosphorus (Pi) is often the primary limiting nutrient in freshwater ecosystems. Since Pi-limitation affects energy transduction, and inorganic carbon (Ci) acquisition can be energy demanding, Ci-acquisition strategies were compared in four species of green algae grown under Pi-replete and Pi-limited conditions predominantly at low and partly at high CO2. Although Pi-limitation was evident by the 10-fold higher cellular C:P ratio and enhanced phosphatase activity, it only decreased Ci-acquisition to a small extent. Nonetheless, the effects of Pi-limitation on both CO2 and HCO3- acquisition were demonstrated. Decreased CO2 acquisition under conditions of Pi limitation was mainly visible in the maximum uptake rate (Vmax) and, for the neutrophile Scenedesmus vacuolatus, in the affinity for CO2 acquisition. Discrimination against 13C was higher under Pi-limited, high CO2 conditions, compared with Pi-replete, high CO2 conditions, in Chlamydomonas acidophila and S. vacuolatus. In the pH-drift experiments, HCO3- acquisition was reduced in Pi-limited C. reinhardtii. In general, energy demanding bicarbonate uptake was indicated by the less strong discrimination against 13C under low CO2 conditions in the neutrophiles (HCO3- users), separating them from the acidophilic or acidotolerant species (CO2 users). The high variability of the influence of Pi supply among different green algal species is linked to their species-specific Ci-acquisition strategies.

Item Type: Publication - Article
Digital Object Identifier (DOI):
UKCEH and CEH Sections/Science Areas: Parr
ISSN: 1916-2790
Additional Keywords: CCM, Chlamydomonas, inorganic carbon uptake kinetics, inorganic phosphorus limitation, stable carbon isotope discrimination
NORA Subject Terms: Biology and Microbiology
Date made live: 27 Sep 2017 14:03 +0 (UTC)

Actions (login required)

View Item View Item

Document Downloads

Downloads for past 30 days

Downloads per month over past year

More statistics for this item...