Single cell microbial ecophysiology with Raman-FISH

Read, Daniel ORCID:; Huang, Wei E.; Whiteley, Andrew S.. 2016 Single cell microbial ecophysiology with Raman-FISH. In: McGenity, Terry J.; Timmis, Kenneth N.; Nogales, Balbina, (eds.) Hydrocarbon and lipid microbiology protocols: single-cell and single-molecule methods. Berlin, Springer, 65-76. (Springer Protocols Handbooks).

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The ability to identify and characterise the roles that bacteria perform in their natural environment is a central prerequisite for understanding how ecosystems function. Traditional methods of culturing and identification are not always suitable due to the inability to grow most bacteria in pure cultures, the so-called great plate count anomaly. Recent developments in culture-independent molecular methods, coupled to microscopy-based ecophysiological analyses, are gaining increasing interest due to their ability to circumvent culture-based biases and allow physiological/phylogenetic analysis within ecological communities. Here we describe the application of Raman microspectroscopy and fluorescence in situ hybridisation (FISH) in combination with stable isotope labelling to help determine bacterial identity and function.

Item Type: Publication - Book Section
Digital Object Identifier (DOI):
UKCEH and CEH Sections/Science Areas: Rees (from October 2014)
ISBN: 9783662491294
Additional Keywords: FISH, fluorescent in situ hybridisation, Raman spectroscopy, stable isotope probing
NORA Subject Terms: Ecology and Environment
Biology and Microbiology
Date made live: 29 Jul 2016 13:13 +0 (UTC)

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