Novel virus discovery and genome reconstruction from field RNA samples reveals highly divergent viruses in dipteran hosts
Cook, Shelley; Chung, Betty Y.-W.; Bass, David; Moureau, Gregory; Tang, Shuoya; McAlister, Erica; Culverwell, C. Lorna; Glücksman, Edvard; Wang, Hui; Brown, T. David K.; Gould, Ernest A.; Harbach, Ralph E.; de Lamballerie, Xavier; Firth, Andrew E.. 2013 Novel virus discovery and genome reconstruction from field RNA samples reveals highly divergent viruses in dipteran hosts. PLoS ONE, 8 (11), e80720. 22, pp. 10.1371/journal.pone.0080720
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Abstract/Summary
We investigated whether small RNA (sRNA) sequenced from field-collected mosquitoes and chironomids (Diptera) can be used as a proxy signature of viral prevalence within a range of species and viral groups, using sRNAs sequenced from wild-caught specimens, to inform total RNA deep sequencing of samples of particular interest. Using this strategy, we sequenced from adult Anopheles maculipennis s.l. mosquitoes the apparently nearly complete genome of one previously undescribed virus related to chronic bee paralysis virus, and, from a pool of Ochlerotatus caspius and Oc. detritus mosquitoes, a nearly complete entomobirnavirus genome. We also reconstructed long sequences (1503-6557 nt) related to at least nine further viruses. Crucially, several of the sequences detected were reconstructed from host organisms highly divergent from those in which related viruses have been previously isolated or discovered. It is clear that viral transmission and maintenance cycles in nature are likely to be significantly more complex and taxonomically diverse than previously expected.
Item Type: | Publication - Article |
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Digital Object Identifier (DOI): | 10.1371/journal.pone.0080720 |
Programmes: | CEH Topics & Objectives 2009 - 2012 > Biodiversity > BD Topic 2 - Ecological Processes in the Environment > BD - 2.2 - Quantify the impact of invasive species, pathogens ... |
UKCEH and CEH Sections/Science Areas: | Reynard |
ISSN: | 1932-6203 |
Additional Information. Not used in RCUK Gateway to Research.: | Open Access paper - Official URL link provides full text |
NORA Subject Terms: | Ecology and Environment Biology and Microbiology |
Date made live: | 26 Nov 2013 16:07 +0 (UTC) |
URI: | https://nora.nerc.ac.uk/id/eprint/503997 |
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