Impact of antibiotic treatment for pulmonary exacerbations on bacterial diversity in cystic fibrosis

Daniels, T.W.V.; Rogers, G.B.; Stressmann, F.A.; van der Gast, C.J.; Bruce, K.D.; Jones, G.R.; Connett, G.J.; Legg, J.P.; Carroll, M.P.. 2013 Impact of antibiotic treatment for pulmonary exacerbations on bacterial diversity in cystic fibrosis. Journal of Cystic Fibrosis, 12 (1). 22-28. https://doi.org/10.1016/j.jcf.2012.05.008

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Abstract/Summary

Background: A diverse array of bacterial species is present in the CF airways, in addition to those recognised as clinically important. Here, we investigated the relative impact of antibiotics, used predominantly to target Pseudomonas aeruginosa during acute exacerbations, on other non-pseudomonal species. Methods: The relative abundance of viable P. aeruginosa and non-pseudomonal species was determined in sputa from 12 adult CF subjects 21, 14, and 7 days prior to antibiotics, day 3 of treatment, the final day of treatment, and 10–14 days afterwards, by T-RFLP profiling. Results: Overall, relative P. aeruginosa abundance increased during antibiotic therapy compared to other bacterial species; mean abundance pre-antibiotic 51.0 ± 36.0% increasing to 71.3 ± 30.4% during antibiotic (ANOVA: F1,54 = 5.16; P < 0.027). Further, the number of non-pseudomonal species detected fell; pre-antibiotic 6.0 ± 3.3 decreasing to 3.7 ± 3.3 during treatment (ANOVA: F1,66 = 5.11; P < 0.027). Conclusions: Antibiotic treatment directed at P. aeruginosa has an additional significant impact on non-pseudomonal, co-colonising species.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1016/j.jcf.2012.05.008
Programmes:	CEH Topics & Objectives 2009 - 2012 > Biodiversity > BD Topic 2 - Ecological Processes in the Environment > BD - 2.3 - Identify indicators and traits of ecosystem status ... CEH Topics & Objectives 2009 - 2012 > Biodiversity > BD Topic 3 - Managing Biodiversity and Ecosystem Services in a Changing Environment > BD - 3.1 - Develop methods to quantify the link between biodiversity .. CEH Topics & Objectives 2009 - 2012 > Biodiversity > BD Topic 2 - Ecological Processes in the Environment
UKCEH and CEH Sections/Science Areas:	Acreman
ISSN:	1569-1993
Additional Keywords:	antibiotics, pulmonary exacerbation, bacterial diversity, T-RFLP, relative abundance
NORA Subject Terms:	Health
Date made live:	27 Sep 2013 11:29 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/503265

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