Dual mutations in the Autographa californica nucleopolyhedrovirus FP-25 and p35 genes result in plasma-membrane blebbing in Trichoplusia ni cells

Kelly, Barbara J.; King, Linda A.; Possee, Robert D.; Chappl, Susan D.J.. 2006 Dual mutations in the Autographa californica nucleopolyhedrovirus FP-25 and p35 genes result in plasma-membrane blebbing in Trichoplusia ni cells. Journal of General Virology, 87. 531-536. https://doi.org/10.1099/vir.0.81312-0

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Abstract/Summary

Spodoptera frugiperda cells infected with Autographa californica nucleopolyhedrovirus (AcMNPV) lacking a functional anti-apoptotic p35 protein undergo apoptosis. However, such mutants replicate normally in Trichoplusia ni (TN-368) cells. An AcMNPV plaque isolate (AcdefrT) was identified during propagation of a virus deficient in p35 in TN-368 cells. This virus exhibited enhanced budded-particle formation in TN-368 cells, but was partially defective for polyhedra production in the same cells. Virus replication in AcdefrT-infected TN-368 cells was accompanied by extensive plasma-membrane blebbing and caspase activation late in infection, both features of apoptosis. Rescue of the p35 locus of AcdefrT continued to result in a reduction in polyhedra and increase in budded virus production in TN-368 cells, but no plasma-membrane blebbing was observed. The mutation was mapped to the FP-25 gene locus. This gene mutation combined with the non-functional p35 was found to be responsible for the cell-blebbing effect observed in AcdefrT-infected TN-368 cells.

Item Type:	Publication - Article
Digital Object Identifier (DOI):	https://doi.org/10.1099/vir.0.81312-0
Programmes:	CEH Programmes pre-2009 publications > Other
UKCEH and CEH Sections/Science Areas:	_ Molecular Virology & Parasitology
ISSN:	0022-1317
Format Availability:	Electronic, Print
NORA Subject Terms:	Biology and Microbiology
Date made live:	04 Jul 2007 11:33 +0 (UTC)
URI:	https://nora.nerc.ac.uk/id/eprint/383

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